T17917

Tricina

≥99% (titration), reagent grade, crystalline

• Sinônimo(s): N-[Tris(hidroximetil)metil]glicina
• Fórmula linear: (HOCH₂)₃CNHCH₂CO₂H
• Número CAS: 5704-04-1
• Peso molecular: 179.17
• Beilstein: 1937804
• Número MDL: MFCD00004277
• Código UNSPSC: 12161700
• NACRES: NA.25

Propriedades

• fonte biológica: synthetic
• Nível de qualidade: 200
• grau: reagent grade
• Ensaio: ≥99% (titration)
• Formulário: crystalline
• condição de armazenamento: (Tightly closed. Dry)
• Impurezas: <0.5% water
• cor: white
• faixa de pH útil: 7.4-8.8
• pKa (25 °C): 8.1
• pf: 187 °C
• solubilidade: water: 0.1 M, clear, colorless
• traços de cátion: Pb: ≤5 ppm
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• chave InChI: SEQKRHFRPICQDD-UHFFFAOYSA-N

Descrição

Descrição geral

Tricine, a zwitterionic biological buffer, is commonly employed in research applications such as enzyme assays, electrophoresis, cell biology, and biochemical studies. Its increased negative charge compared to glycine ensures rapid migration during electrophoresis, facilitating efficient separation of low-molecular-weight proteins in gels with lower acrylamide percentages. Moreover, Tricine may also be used in cryopreservation media, aiding in the preservation of tissues and organs.

Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.

Aplicação

Componente de tampão para separação de peptídeos de baixo peso molecular.

Tricine is intended to use for:

• Electrophoresis To separate low-weight peptides and proteins
• Crystallization of proteins of varying pH
• Resuspension of cell pellets
• Enzyme assays
• Tissue culture

Características e benefícios

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7.4 - 8.8 with a pKa of 8.1 (25 °C)

Outras notas

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Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable