SBR00028
FITC labeled vancomycin
for fluorescent microbial imaging
Sinônimo(s):
Vancomycin -FITC, Vancomycin FITC conjugate
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About This Item
Fórmula empírica (Notação de Hill):
C87H88Cl2N10O29S
Peso molecular:
1840.65
Código UNSPSC:
51102829
NACRES:
NA.76
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Nível de qualidade
Ensaio
≥85% (HPLC)
forma
solid
peso molecular
Mw 1840.66 g/mol
disponibilidade
not available in China
condição de armazenamento
protect from light
cor
yellow to dark orange
adequação
suitable for (Fluorescent detection of gram-positive bacteria)
suitable for (Microbiome)
suitable for microbiology
espectro de atividade do antibiótico
Gram-positive bacteria
Modo de ação
DNA synthesis
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Categorias relacionadas
Descrição geral
FITC labeled Vancomycin (Vancomycin-FITC), is a Fluorescein conjugated Vancomycin, with a similar mode of action as Vancomycin. Vancomycin -FITC can be used to detect Gram-positive bacteria by common fluorescent instruments. Vancomycin is a glycopeptide antibiotic, used to treat gram-positive infections. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore.
Aplicação
Fluorescent antibiotics can be used for many applications including:
- Antimicrobial resistance research.
- Bacterial visualization and imaging.
- Parent antibiotic mode of action research and new antibiotic discovery.
- Toxicity studies.
- Research of bacterial infections and tracking its uptake in vivo.
Ações bioquímicas/fisiológicas
Vancomycin inhibits cell wall synthesis at the level of peptidoglycan biosynthesis. Vancomycin binds with high affinity and specificity to the terminal D-Ala-D-Ala of the growing peptide chain during cell wall synthesis. The binding inhibits transpeptidase activity, which prevents further elongation and cross-linking of the peptidoglycan matrix. Vancomycin-FITC has fluorescein attached to the amine group on the disaccharide moiety of the vancomycin molecule. This disaccharide moiety does not play any role in D-Ala-D-Ala binding site, affording a comparable binding affinity of Vancomycin-FITC to Gram-positive bacteria as the parent drug. However, a minimum inhibitory concentration (MIC) decrease of about two orders of magnitude in comparison to Vancomycin was observed. This activity decrease can be linked to repulsive interactions between the negatively charged Fluorescein moiety and the anionic environment of the peptidoglycan.
Características e benefícios
- High-quality antibiotic suitable for multiple research applications
- Ideal for immunological, biochemical, and cell biology applications
- Suitable for fluorescent microscopy imaging
Nota de análise
- Aliquots of the DMSO solution can be stored at -20°C, protected from light for at least 1 month.
- Fluorescent microscopy application: Vancomycin -FITC has excitation/emission wavelength at 495 nm/515 nm.
- It is recommended to dissolve Vancomycin-FITC in DMSO (Dimethyl Sulfoxide) to a concentration of 1mg/mL.
- Recommended working concentration for Bacillus cereus in fluorescent microscopy imaging application is 25μg/mL.
Outras notas
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Analytical techniques for vancomycin−A review.
Sattur A P, et al.
Biotechnology and Bioprocess Engineering: BBE, 5, 153-158 (2000)
P J Loll et al.
Annual review of biophysics and biomolecular structure, 29, 265-289 (2000-08-15)
Vancomycin is the archetype among naturally occurring compounds known as glycopeptide antibiotics. Because it is a vital therapeutic agent used world-wide for the treatment of infections with gram-positive bacteria, emerging bacterial resistance to vancomycin is a major public health threat.
Richard A Daniel et al.
Cell, 113(6), 767-776 (2003-06-18)
Cell shape in most eubacteria is maintained by a tough external peptidoglycan cell wall. Recently, cell shape determining proteins of the MreB family were shown to form helical, actin-like cables in the cell. We used a fluorescent derivative of the
Pedro M Pereira et al.
Antimicrobial agents and chemotherapy, 51(10), 3627-3633 (2007-07-25)
A new method of fluorescence ratio imaging microscopy was used to compare the in vivo binding capacity and the access of a fluorescent derivative of vancomycin to the cell wall synthetic sites in isogenic pairs of vancomycin-susceptible and -resistant laboratory
Kittichoat Tiyanont et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(29), 11033-11038 (2006-07-13)
The peptidoglycan (PG) layers surrounding bacterial cells play an important role in determining cell shape. The machinery controlling when and where new PG is made is not understood, but is proposed to involve interactions between bacterial actin homologs such as
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