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Merck
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Documentos

17170

Millipore

AEA Sporulation Broth (Base), modified

NutriSelect® Plus, suitable for microbiology

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About This Item

Código UNSPSC:
41171606
NACRES:
NA.85

esterilidade

non-sterile

Nível de qualidade

forma

powder

prazo de validade

limited shelf life, expiry date on the label

composição

ammonium acetate, 1.5 g/L
biopeptone, 10 g/L
disodium phosphate, 4.36 g/L
magnesium sulfate.7H2O, 0.2 g/L
monopotassium phosphate, 0.25 g/L
yeast extract, 10 g/L

fabricante/nome comercial

NutriSelect® Plus

técnica(s)

microbiological culture: suitable

pH final

7.8±0.2 (25 °C)

aplicação(ões)

food and beverages
microbiology

adequação

nonselective for Clostridium spp.

Aplicação

For early sporulation of Clostridium perfringens from foods.

Nota de preparo

Suspend 26.31 g in 1 litre distilled water. Dispense the medium in 15 ml amounts in screw capped tubes and sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and add 0.6 ml of filter sterilized 10% raffinose and 0.2 ml each of sterile 0.66 M sodium carbonate and 0.32% cobalt chloride dropwise to each 15 ml base medium in the tubes. Just before use, steam the medium for 10 minutes and after cooling, add 0.2 ml of filter sterilized (freshly prepared) 1.5% sodium ascorbate to each tube of the medium.

Nota de rodapé

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informações legais

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Joyshree Ganguly et al.
Anaerobe, 63, 102208-102208 (2020-05-11)
Single-cell analysis of microbial population heterogeneity is a fast growing research area in microbiology due to its potential to identify and quantify the impact of subpopulations on microbial performance in, for example, industrial biotechnology, environmental biology, and pathogenesis. Although several

Artigos

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.

Clostridium perfringens can cause contamination in undercooked or improperly sterilized canned foods and water. Learn to detect, identify, and differentiate this pathogen.

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