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114391

Sigma-Aldrich

Bromophenol Blue

ACS reagent

Sinônimo(s):

3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form

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About This Item

Fórmula empírica (Notação de Hill):
C19H10Br4O5S
Número CAS:
115-39-9
Peso molecular:
669.96
Beilstein:
61698
Número CE:
204-086-2
Número MDL:
MFCD00005875
Código UNSPSC:
12171500
ID de substância PubChem:
24847169
NACRES:
NA.47

grau

ACS reagent

Nível de qualidade

200

Formulário

powder

técnica(s)

titration: suitable

visual transition interval

3.0-4.6, yellow to blue

pf

273 °C (lit.)

solubilidade

ethanol: 0.1%

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

chave InChI

UDSAIICHUKSCKT-UHFFFAOYSA-N

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Descrição geral

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Aplicação

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
SHBR7468
BCCM1010
SHBR1340
BCCJ9036
BCCK2682

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