PC35
Anti-p53 (Ab-7) (Pantropic) Sheep pAb
liquid, Calbiochem®
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About This Item
Código UNSPSC:
12352203
clone:
polyclonal
application:
reatividade de espécies:
human, rat, mouse
citations:
14
Produtos recomendados
fonte biológica
sheep
Nível de qualidade
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
Formulário
liquid
contém
≤0.1% sodium azide as preservative (Anti-p53 and Anti-Sheep IgG only)
reatividade de espécies
human, rat, mouse
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
Isotipo
IgG
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... TP53(7157)
Descrição geral
Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 and SVT2 cells and breast carcinoma tissue.
Sheep polyclonal antibody supplied as diluted serum. Recognizes the ~53 kDa wild-type or mutant p53 protein. Supplied with normal sheep serum and biotinylated rabbit anti-sheep IgG.
This Anti-p53 (Ab-7) (Pantropic) Sheep pAb is validated for use in Frozen Sections, Immunoblotting, ICC, Paraffin Sections, IP for the detection of p53 (Ab-7) (Pantropic).
Imunogênio
Human
recombinant, human p53 protein
Aplicação
Frozen Sections (1:500)
Immunoblotting (1:2500)
Immunocytochemistry (1:500)
Paraffin Sections (1:500, pepsin or heat pre-treatment required)
Immunoprecipitation (chromatin, see application references)
Componentes
Anti-p53 antisera, normal sheep serum, and biotinylated rabbit anti-sheep IgG
Advertência
Toxicity: Standard Handling (A)
forma física
200 µl diluted (1:4) anti-p53 sheep serum, 500 µl undiluted normal sheep serum, and 100 µl biotinylated rabbit anti-sheep IgG.
Nota de análise
Positive Control
A431 or SVT2 cells or breast carcinoma tissue
A431 or SVT2 cells or breast carcinoma tissue
Outras notas
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Recommended dilutions for the biotinylated rabbit anti-sheep secondary are 1:25,000-1:200,000 for immunoblotting and 1:10,000 for immunocytochemistry and paraffin sections. For immunoblotting, do not add normal serum, non-fat dried milk, culture medium or other potential sources of biotin to the working solutions (i.e. block with 2-5% fatty acid free BSA). Use streptavidin-horseradish peroxidase (Cat. No. OR03L) or streptavidin-alkaline phosphatase (Cat. No. OR04L) at 10 ng/ml to attach an enzyme to the biotinylated secondary antibody, then visualize using standard colorimetric or chemiluminescent reagents. To maximize sensitivity of immunoblots, preconcentrate samples by immunoprecipitation with Cat. No. OP03, OP09, or OP43, then immunoblot using Cat. No. PC35 and chemiluminescent detection. Antibodies should be titrated for optimal results in individual systems.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
10 - Combustible liquids
Certificados de análise (COA)
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Vashe Chandrakanthan et al.
Reproductive biology and endocrinology : RB&E, 4, 61-61 (2006-11-23)
In the mouse, embryo culture results in a characteristic phenotype of retarded embryo preimplantation development and reduced numbers of cells within embryos. The expression of TRP53 is central to the regulation of the cell's capacity to proliferate and survive. In
Kenneth A Giuliano
Methods in molecular biology (Clifton, N.J.), 356, 189-193 (2006-09-22)
Immunoreagents formed the basis of early fixed end point high content screening (HCS) assays and their use in HCS applications in drug discovery will continue to increase. One important application of immunoreagents is their incorporation into multiplexed HCS assays in
Timothy A Sutton et al.
American journal of physiology. Renal physiology, 295(6), F1666-F1677 (2008-09-26)
Ischemia-reperfusion injury (IRI) is a common cause of acute kidney injury (AKI) and is characterized by widespread tubular and microvascular damage. The tumor suppressor p53 is upregulated after IRI and contributes to renal injury in part by promoting apoptosis. Acute
M Ciciarello et al.
The Journal of biological chemistry, 276(22), 19205-19213 (2001-05-29)
Growing evidence indicates a central role for p53 in mediating cell cycle arrest in response to mitotic spindle defects so as to prevent rereplication in cells in which the mitotic division has failed. Here we report that a transient inhibition
Joshua D Dowell et al.
Biochimica et biophysica acta, 1773(3), 358-366 (2007-01-19)
p193/CUL7 is an E3 ubiquitin ligase initially identified as an SV40 Large T Antigen binding protein. Expression of a dominant interfering variant of mouse p193/CUL7 (designated 1152stop) conferred resistance to MG132- and etoposide-induced apoptosis in U2OS cells. Immune precipitation/Western analyses
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