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MAIPN4550

Millipore

Placa Multiscreen® de 96 poços, membrana hidrofóbica de PVDF

pore size 0.45 μm, non-sterile

Sinônimo(s):

Placa PVDF de 96 poços, Placa de membrana PVDF, Placa hidrofóbica de 96 poços

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About This Item

Código UNSPSC:
41104923
eCl@ss:
32039006
NACRES:
NB.22
Preço e disponibilidade não estão disponíveis no momento.

Materiais

PVDF membrane (hydrophobic)
acrylic
flat bottom wells

Nível de qualidade

400

descrição

Non-sterile, clear 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for Avidin-biotin Linkages, DNA-binding Proteins, Protein Binding & PAMPA. Comes in a pack of 50.

esterilidade

non-sterile

linha de produto

MultiScreen®

Características

hydrophobic
lid

fabricante/nome comercial

MultiScreen®

Parâmetros

50-250 μL sample volume (per well)

técnica(s)

parallel artificial membrane permeation assay (PAMPA): suitable

área de filtração

0.3 cm2

tamanho da placa

96 wells

volume máximo do poço

300 μL

volume de trabalho

50-250 μL

Matriz

Immobilon®-P

tamanho de poro

0.45 μm pore size

tipo de ligação

high binding surface

Condições de expedição

ambient

Categorias relacionadas

Aplicação

A placa de filtro MultiScreen®-IP, 0,45 μm, transparente, não estéril foi usada:
  • como suporte de membrana artificial e placa receptora em ensaio de permeabilidade em membrana artificial paralela (PAMPA) para determinar as permeabilidades de oximas[1]
  • em ensaio ELISpot (do inglês, enzyme-linked immunospot)[2]
  • em ELISPOT específico para hemocianina de lapa californiana (KLH) para a identificação de células específicas de antígenos (Ag)[3]

Informações legais

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Claudia Giesecke et al.
Journal of immunology (Baltimore, Md. : 1950), 200(12), 3981-3992 (2018-05-08)
There are currently limited insights into the progression of human primary humoral immunity despite numerous studies in experimental models. In this study, we analyzed a primary and related secondary parenteral keyhole limpet hemocyanin (KLH) immunization in five human adults. The
A C Belkina et al.
Journal of dental research, 99(7), 855-862 (2020-03-19)
Periodontitis (PD) is a common source of uncontrolled inflammation in obesity-associated type 2 diabetes (T2D). PD apparently fuels the inflammation of T2D and associates with poor glycemic control and increased T2D morbidity. New therapeutics are critically needed to counter the
Hee Chun Jeong et al.
Bioorganic & medicinal chemistry, 17(17), 6213-6217 (2009-08-12)
A series of fluorinated oxime compounds was designed and synthesized in order to probe the effect of fluorine substitution on reactivation of inhibited acetylcholinesterase (AChE) by organophosphorus agents. Permeability measurements, using the Parallel Artificial Membrane Permeation Assays (PAMPA) method, were

Artigos

Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA)

This is a white paper on the Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA) using MultiScreen® Filter Plates.

Membrane Integrity Test for Lipid-PAMPA Artificial Membranes

This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes

Permeability & Solubility Analysis Workflow

Combining solubility & PAMPA assays streamlines drug permeability testing.

Protocolos

HDM-PAMPA with the MultiScreen Permeability Filter Plate

Assays that predict passive absorption of orally administered drugs have become increasingly important in the drug discovery process. As previously described by Faller and Kansy such assays provide rapid, low cost and automation friendly methods to measure a compound’s passive permeability.

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

Questions

  1. Could you please explain the meaning of the terms "donor," "acceptor," "receiver," and "collection" in relation to MAIPN4550 and MAIPN4510 filter plates?

    1 answer

    1. The donor plate is also known as the filter plate, while the acceptor plate can be referred to as the acceptor, receiver, or collection plate. For detailed information on the PAMPA assay, you can refer to the provided link. Additionally, a datasheet is available that reviews some of the donor and acceptor plates. The donor plates include MAIPN4550 and MAIPNTR10. An alternative donor plate option is S2EM004M99, which is a 'MA' classic designed plate. It is important to note that any plate starting with 'MS---' in the catalog number cannot be used.

      As for the acceptor plates, several options are available, such as MSSACCEPT0R, MATRNPS50, and MSCPNUV40.

      Moreover, tech notes for the automation of PAMPA-Lipid on the Sciclone ALH 3000 workstation and the automation of PAMPA-HDM using a MultiScreen® Filter Plate on a Beckman Biomek® FX Workstation are also available. An article titled "Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA)" provides further insights into the topic.

      Helpful?

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