MABS465

Anti-Nampt, clone 14A.5 Antibody

clone 14A5, from rat

• Sinônimo(s): PBEF, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1 homolog, PBEF, Visfatin
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rat
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 14A5, monoclonal
• reatividade de espécies: human, mouse
• técnica(s): ELISA: suitable; immunohistochemistry: suitable; inhibition assay: suitable; western blot: suitable
• Isotipo: IgG2aκ
• nº de adesão NCBI: NP_067499
• nº de adesão UniProt: Q99KQ4
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... NAMPT(10135)

Descrição

Descrição geral

Nicotinamide phosphoribosyltransferase (NAMPT) is a member of the NAPRTase family. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. NAMPT is localized in the cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis.

Imunogênio

Recombinant protein corresponding to mouse Nampt.

Aplicação

Anti-Nampt antibody, clone 14A.5 is an antibody against Nampt for use in western blotting, IHC, inhibition & ELISA.

Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Nampt in Anti-CD3epsilon stimulated mouse spleen cell lysate (Prof. Oberdan Leo, Universite Libre de Bruxelles.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Nampt in mouse spleen tissue lysate (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

Inhibition Analysis: A representative lot from an independent laboratory was inhibited by a synthetic peptide corresponding to murine Nampt in total liver extracts (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Nampt in CD11c positive mouse spleen sections (Prof. Oberdan Leo, Universite Libre de Bruxelles.).

ELISA Analysis: A representative lot from an independent laboratory detected recombinant Nampt peptides (Ronveaux, A., et al. (2002). Eur J Immunol. 32(11):3225-3234.).

Qualidade

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nampt in 10 µg of Raji cell lysate.

Descrição-alvo

~56 kDa observed

forma física

Format: Purified

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable