MABS1326

Anti-Importin alpha-3/Qip1 Antibody, clone 3D10

clone 3D10, from rat

• Sinônimo(s): Importin subunit alpha-3, Importin alpha Q1, Karyopherin subunit alpha-4, Qip1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rat
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 3D10, monoclonal
• reatividade de espécies: rat, monkey, hamster, bovine, human, mouse
• reatividade da espécie (prevista por homologia): canine (based on 100% sequence homology)
• técnica(s): western blot: suitable
• Isotipo: IgG2aκ
• nº de adesão NCBI: NP_032493
• nº de adesão UniProt: O35343
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: bovine ... Kpna3(540719) ; dog ... Kpna3(476909) ; human ... KPNA3(3839) ; mouse ... Kpna3(16648) ; rat ... Kpna3(361055) ; rhesus monkey ... Kpna3(706925)

Descrição

Descrição geral

Importin subunit alpha-3 (UniProt O35343; also known as Importin alpha Q1, Karyopherin subunit alpha-4, Qip1) is encoded by the Kpna4 (also known as Qip1) gene (Gene ID 16649) in murine species. The transport of macromolecules between cytoplasm and nucleus is mediated by importins and exportins and regulated by the small GTPase Ran. Importins bind their cargoes with either nuclear localization signal (NLS) or M9 domain-containing hnRNP proteins in the cytoplasm, while exportins target their substrates via their nuclear export signal (NES) or M9 domain inside the nucleus. Qip1/KPNA4 belongs to the importin- /karyopherin- (KPNA) family of adaptor proteins that interact with both monopartite and bipartite types of classical nuclear localization signals (cNLSs). The seven KPNA family members are divided into the 1 (KPNA1/5/6), 2 (KPNA2/7), and 3 (KPNA3/4) subfamilies based on structural and functional similarities. KPNA members mediate substrate cargo nuclear import by bridging substrate cargo and the Ran-karyopherin- complex. Both KPNA3 and Qip1/KPNA4 are reported to target methyl-CpG-binding protein 2 (MeCp2) bipartite NLS (255-RKAEADPQAIPKKRGRK-271). Qip1/KPNA4 can ineract with both wild-type and MeCp2 R270X mutants found in Rett syndrome (RTT) patients, while KPNA3 binds only wild-type, but not MeCp2 R270X mutants. Human and murine Qip1 contains an NLS (a.a. 43-52) in its importin- -binding (IBB) domain (a.a. 2-58), followed by ten ARM domains (a.a. 66-485), one majore and one minor NLS-binding site (a.a. 137-229 and a.a. 306-394).

Especificidade

Clone 3D10 detected recombinant mouse importin 3/Qip1, but not importin 1/Rch1, importin 5/NPI-1, or importin 7/S2 GST fusion proteins (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400).

Imunogênio

Recombinant full-length mouse Importin alpha-3/Qip1.

Aplicação

Detect Importin subunit alpha-3/Qip1 using this rat monoclonal Anti-Importin alpha-3/Qip1, clone 3D10, Cat. No. MABS1326, validated for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected recombinant mouse importin 3/Qip1, but not importin 1/Rch1, importin 5/NPI-1, or importin 7/S2 GST fusion proteins (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400).

Western Blotting Analysis: A representative lot detected endogenous importin 3/Qip1 in human HeLa, monkey COS-7, mouse L-929, rat NRK , hamster BHK, and bovine MDBK cell lysates (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400).

Qualidade

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected in 10 µg of HeLa cell lysate.

Descrição-alvo

~58 kDa observed. 57.76 kDa (human) and 57.79 kDa (mouse & rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified

Protein G purified.

Purified rat IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable