MABE432

Anti-TRIM33 Antibody, clone 3B5.1

clone 3B5.1, 1 mg/mL, from mouse

• Sinônimo(s): E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, Protein Rfg7, Transcription intermediary factor 1-gamma, TIF1-gamma, Tripartite motif-containing protein 33
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 3B5.1, monoclonal
• reatividade de espécies: human
• concentração: 1 mg/mL
• técnica(s): immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_056990
• nº de adesão UniProt: Q9UPN9
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... TRIM33(51592)

Descrição

Descrição geral

TRIM33, also known as E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, Rfg7, Transcription intermediary factor 1-gamma, TIF1-gamma, or Tripartite motif-containing protein 33, and encoded by the gene TRIM33/KIAA1113/RFG7/TIF1G, is an E3 ubiquitin protein ligase. Among its targets is SMAD4 for which ubiquitination facilitates its nuclear exclusion and degradation. SMAD3 is also a target for ubiquitination, and together, TRIM33′s association promotes erythroid differentiation of hematopoietic stem cells. Finally mono-ubiquitination of various targets by TRIM33 affects their ability to form complexes which often hampers their activity and thus regulates their subsequent actions. Such is the case of SMAD4 and its interaction with TGF-beta/BMP signaling. Monoubiquitination of SMAD4 by TRIM33 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. TRIM33 is localized in nucleus in nuclear dots or bodies and is expressed in most tissues and stem cells during development as well as in the adult in multiple tissues.

Imunogênio

GST-tagged recombinant protein corresponding to human TRIM33.

Aplicação

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected TRIM33 in H9 stem cells.
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Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-TRIM33 Antibody, clone 3B5.1 is validated for use in Western Blotting and Immunocytochemistry for the detection of TRIM33.

Qualidade

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected TRIM33 in 10 µg of K562 cell lysate.

Descrição-alvo

~123 kDa observed. Uncharacterized band(s) may appear in some lysates.

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Informações legais

ALEXA FLUOR is a trademark of Life Technologies

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable