MAB2226

Anti-Piccolo Antibody, clone 4G3.3

clone 4G3.3, from mouse

• Sinônimo(s): presynaptic cytomatrix protein, Aczonin
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 4G3.3, monoclonal
• reatividade de espécies: mouse
• reatividade da espécie (prevista por homologia): human (based on 82% homology), rat (92% homology)
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgMκ
• nº de adesão NCBI: NP_001104266
• nº de adesão UniProt: Q9Y6V0
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PCLO(27445) ; mouse ... Pclo(26875)

Descrição

Descrição geral

Piccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE].

Especificidade

This antibody recognizes piccolo.

Imunogênio

GST-tagged recombinant protein

Aplicação

Immunohistochemistry (Paraffin):
Representative images from a previous lot.
Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

This Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo.

Qualidade

Routinely evaluated by western blot on rat brain lysate.

Descrição-alvo

547 kDa

forma física

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS and 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Rat brain lysate, mouse cerebellum

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable