GF173

Noggin Protein, Human Recombinant

The human Noggin recombinant protein belongs to a group of diffusible proteins which bind to ligands of the TGF-β family & regulate their activity by inhibiting their access to signaling receptors.

• Sinônimo(s): NOG, SYM1, SYNS1
• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

Propriedades

• recombinante: expressed in HEK 293 cells
• Nível de qualidade: 100
• Ensaio: >95% (SDS-PAGE and HPLC)
• Impurezas: <0.1 μg/mg protein endotoxin (<1EU/μg)
• nº de adesão UniProt: Q13253
• Condições de expedição: dry ice

Descrição

Descrição geral

Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. The interplay between TGF-β ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant human Noggin is a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains. Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS PAGE analysis under reducing conditions.

Product Source: HEK293 cells

Especificidade

Cross Reactivty
Mouse, Chicken

Aplicação

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Qualidade

Bioactivity assay: Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.

forma física

Product is filtered through a 0.2 micron filter before lyophilization.

Armazenamento e estabilidade

Store at -20°C for up to 6 months from date of receipt.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable