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EZHTNFA-150K

Millipore

Human TNF-α ELISA Kit

detects TNF-α in 50 μL serum, plasma or cell culture supernatent

Sinônimo(s):

Human TNF-A ELISA, TNF-A ELISA, TNF-Alpha, Tumour Necrosis Factor Alpha ELISA

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.77

product name

Human TNF-α Conferma® ELISA Kit, kit sufficient for 96 wells

Nível de qualidade

reatividade de espécies

human

embalagem

kit of 1 × 96 wells

Parâmetros

50 μL sample volume (4hr assay)

assay range

linearity: 89-113%
(Serum, 101%)

linearity: 98-110%
(Plasma, 103%)

recovery range: 81-111%
(Plasma, 97%)

recovery range: 85-105%
(Serum,95%)

inter-assay cv: ≤7%
intra-assay cv: ≤3%
sensitivity: 1.65 pg/mL
standard curve range: 1.65-400 pg/mL

técnica(s)

ELISA: suitable

entrada

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

aplicação(ões)

research use

método de detecção

colorimetric (450nm/590nm)

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

TNF-α is a core biomarker for the study of inflammation, considered to be a major contributing factor to numerous diseases and pathological processes. The Conferma® ELISA for TNF-α uses highly characterized critical reagents assessed by LC-MS, RPLC-UV-MS and Surface Plasmon Resonance (SPR) to deliver ensure similarity and that the mAb / Standard relationship is maintained . Each assay is further anchored against a recombinant gold standard and a sample library to ensure strong finished kit performance lot to lot. Detailed information can be found in the Protocol & Assay Verification Report.

Research Category: Inflammation

Research Sub-Category:
Alzheimer′s Disease
Aging
Inflammation
Neurodegenerative Diseases
Metabolic Disease
Sepsis Viral Diseases
Cardiovascular Disease

Especificidade

This assay was tested for cross reactivity with other human cytokines like IL-1a, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17A, CCL3, CCL4, MCP-1, TNFα, GMCSF, VEGF. There is no cross-reactivity detected with any analyte.

Aplicação

For the detection of TNF-α by ELISA in Human Serum & Plasma.

Características e benefícios

Capture ELISA assay. Highly Verified. Suitable for 37 samples in Duplicate, with a 6 point Standard Curve, 3 Quality Controls and a Blank.

Informações legais

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2

Órgãos-alvo

Respiratory Tract

Código de classe de armazenamento

8A - Combustible, corrosive hazardous materials

Classe de risco de água (WGK)

WGK 3


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Artigos

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

Protocolos

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

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