ECM210
QCM Endothelial Cell Invasion Assay (24 well, colorimetric)
This QCM Endothelial Cell Invasion Assay provides an in vitro model to quickly screen factors that can regulate endothelial invasion. The assay is performed in an invasion chamber using a basement membrane protein coated on the porous insert.
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352207
eCl@ss:
32161000
NACRES:
NA.84
Produtos recomendados
Nível de qualidade
fabricante/nome comercial
Chemicon®
QCM
técnica(s)
cell based assay: suitable
método de detecção
colorimetric
Condições de expedição
wet ice
Categorias relacionadas
Descrição geral
Also available: Cell Comb Scratch Assay! Get biochemical data from a scratch assay!Click Here
Introduction
Endothelial cells (EC) invade through the basement membrane (BM) to form sprouting vessels. The invasion process consists of the secretion of matrix metalloproteases (MMP) to degrade basement membrane, the activation of endothelial cells, and the migration of EC across the basement membrane. The understanding of EC invasion is important for studying the mechanism of angiogenesis in injured tissue as well as in disease such as cancer.
Cell migration may be evaluated through several different methods, the most widely accepted of which is the Boyden Chamber assay. The Boyden Chamber system uses two-chamber system which a porous membrane provides an interface between two chambers. Cells are seeded in the upper chamber and chemoattractants placed in the lower chamber. Cells in the upper chamber migrate toward the chemoattractants by passing through the porous membrane to the lower chamber. Migratory cells are then stained and quantified.
Introduction
Endothelial cells (EC) invade through the basement membrane (BM) to form sprouting vessels. The invasion process consists of the secretion of matrix metalloproteases (MMP) to degrade basement membrane, the activation of endothelial cells, and the migration of EC across the basement membrane. The understanding of EC invasion is important for studying the mechanism of angiogenesis in injured tissue as well as in disease such as cancer.
Cell migration may be evaluated through several different methods, the most widely accepted of which is the Boyden Chamber assay. The Boyden Chamber system uses two-chamber system which a porous membrane provides an interface between two chambers. Cells are seeded in the upper chamber and chemoattractants placed in the lower chamber. Cells in the upper chamber migrate toward the chemoattractants by passing through the porous membrane to the lower chamber. Migratory cells are then stained and quantified.
Aplicação
Millipore’s QCM Endothelial Cell Invasion Assay provides an in vitro model to quickly screen factors that can regulate endothelial invasion. The assay is performed in an invasion chamber using a basement membrane protein coated on the porous insert. The level of coating and the pore size is optimized for endothelial cells so the researcher may utilize this kit to mimic physiological condition. After cell invasion has occurred, the researcher can select a staining method to quantify the number of cells that have invaded through the chamber. Millipore offers a colorimetric staining kit (ECM210) and a fluorometric kit staining reagent (ECM211) for both convenience and efficiency.
Research Category
Apoptosis & Cancer
Cell Structure
Apoptosis & Cancer
Cell Structure
Embalagem
Sufficient for 24 assays
Componentes
1. Cell Invasion Plate Assembly: (Part No. CS203020) Two 24-well plates each containing 12 ECMatrix-coated 3 m inserts per plate.
2. Cell Stain Solution: (Part No. 90144)* One bottle.
3. Extraction Buffer: (Part No. 90145) One bottle.
4. 24-well Stain Extraction Plate: (Part No. 2005871) One each.
4. 24-well Stain Extraction Plate: (Part No. 2005871) One each.
5. 96-well Stain Quantitation Plate: (Part No. 2005870) One each.
6. Cotton Swabs: (Part No. 10202) Fifty each.
7. Forceps: (Part No. 10203) One each.
2. Cell Stain Solution: (Part No. 90144)* One bottle.
3. Extraction Buffer: (Part No. 90145) One bottle.
4. 24-well Stain Extraction Plate: (Part No. 2005871) One each.
4. 24-well Stain Extraction Plate: (Part No. 2005871) One each.
5. 96-well Stain Quantitation Plate: (Part No. 2005870) One each.
6. Cotton Swabs: (Part No. 10202) Fifty each.
7. Forceps: (Part No. 10203) One each.
Armazenamento e estabilidade
Store kit materials at 2° to 8°C for up to their expiration date. Do not freeze.
Informações legais
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Flam. Liq. 2
Código de classe de armazenamento
3 - Flammable liquids
Ponto de fulgor (°F)
53.6 °F
Ponto de fulgor (°C)
12 °C
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Os clientes também visualizaram
Yingying Yu et al.
Bioengineered, 12(1), 4681-4696 (2021-08-05)
Accumulating evidence indicates that INHBA (Inhibin β-A, a member of the TGF-β superfamily) functions as an oncogene in cancer progression. However, little is known as to how INHBA regulates the progression and aggressiveness of breast cancer (BC). This study explored
Sylvain Tollis et al.
ACS chemical biology, 17(3), 680-700 (2022-02-25)
Background: Lower survival rates for many cancer types correlate with changes in nuclear size/scaling in a tumor-type/tissue-specific manner. Hypothesizing that such changes might confer an advantage to tumor cells, we aimed at the identification of commercially available compounds to guide
Artigos
Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology.
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica