ABT319

Anti-Nephrin Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Nephrin, Renal glomerulus-specific cell adhesion receptor
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: polyclonal
• application: IHC; WB
• reatividade de espécies: human
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• citations: 1

Propriedades

• fonte biológica: rabbit
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• nº de adesão NCBI: NP_004637
• nº de adesão UniProt: O60500
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... NPHS1(4868)

Descrição

Descrição geral

Nephrin (NPHS1, NPHN), also known as is Renal glomerulus-specific cell adhesion receptor, is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.

Especificidade

This antibody is expected to detect both known isoforms of Nephrin.

Imunogênio

Epitope: NPHS2 binding domain

KLH-conjugated linear peptide corresponding to the NPHS2 binding domain of human Nephrin.

Aplicação

Anti-Nephrin Antibody is a highly specific rabbit polyclonal antibody, that targets Nephrin & has been tested in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Nephrin in human kidney tissue.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Qualidade

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nephrin in 10 µg of human kidney tissue lysate.

Descrição-alvo

~190 kDa and ~85 kDa observed. Two isoforms are known to exist at 134kDa (Isoform 1) and 130kDa (Isoform 2). This protein may be observed at higher molecular weights due to glycosylation (Ruotsalainen, V., et al. (1999). Proc Natl Acad Sci USA. 6(96):7962-7967.).

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable