ABS219

Anti-phospho-Tie2 (Ser1119) Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Angiopoietin-1 receptor, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, hTIE2, p140 TEK, CD202b
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
• técnica(s): western blot: suitable
• nº de adesão NCBI: NP_000450
• nº de adesão UniProt: Q02763
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pSer1119)
• Informações sobre genes: human ... TEK(7010)

Descrição

Descrição geral

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Especificidade

This antibody recognizes Tie2 phosphorylated at Ser1119.

Imunogênio

Epitope: Phosphorylated Ser1119

KLH-conjugated linear peptide corresponding to human Tie2 phosphorylated at Ser1119.

Aplicação

Anti-phospho-Tie2 (Ser1119) Antibody is an antibody against phospho-Tie2 (Ser1119) for use in WB.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Qualidade

Evaluated by Western Blot in HEK293T overexpressing Tie2 protein.

Western Blot Analysis: A 1:500 dilution detected Tie2 on 10 µg of HEK293T overexpressing Tie2 protein.

Descrição-alvo

~160 kDa observed. Uniprot gives a calculated molecular weight of 126 kDa, but has been observed at ~160 kDa due to glycosolation. A non-modified specific band appears at ~52 kDa in some lysates.

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
HEK293T overexpressing Tie2 protein

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable