ABE1304

Anti-monomethyl Histone H3 (Lys4) Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: rat, human
• técnica(s): dot blot: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• nº de adesão NCBI: NP_003484
• nº de adesão UniProt: P68431
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: monomethylation (Lys4)
• Informações sobre genes: human ... HIST1H3F(8968)

Descrição

Descrição geral

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Histone H3 is acetylated on Lys14 by Gcn5, and histone H3 phosphorylation at Ser10 facilitates acetylation of Lys14 by Gcn5 in vitro. Moreover, Lys14 is involved in chromatin remodeling, and RSC4 (part of the RSC chromatin remodeling complex) contains essential tandem bromodomains that require H3 Lys14 acetylation for gene activation.

Imunogênio

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human monomethyl Histone H3 (Lys4).

Aplicação

Anti-monomethyl Histone H3 (Lys4) is an antibody against monomethyl Histone H3 (Lys4) for use in WB, IP, IH, Dot Blot.

Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected monomethyl Histone H3 (Lys4) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl Histone H3 (Lys4) in 500 µg of HeLa acid extract.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected monomethyl Histone H3 (Lys4) in human and rat testis tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Qualidade

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected monomethyl Histone H3 (Lys4) in 10 µg of HeLa acid extract.

Descrição-alvo

~17 kDa observed

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable