AB175

Anti-GABA Antibody

serum, Chemicon^®

• Sinônimo(s): Anti-GABA Receptor Antibody, GABA Receptor Antibody
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: guinea pig
• Nível de qualidade: 100
• forma do anticorpo: serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: rat
• reatividade da espécie (prevista por homologia): all
• fabricante/nome comercial: Chemicon^®
• técnica(s): ELISA: suitable; immunohistochemistry: suitable (paraffin)
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: rat ... Gabra1(29705)

Descrição

Descrição geral

GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA is produced from glutamic acid in a reaction catalyzed by glutamic acid decarboxylase. In this reaction pyridoxal phosphate serves as a co-factor. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. They differ in their pharmacological, electrophysiological, and biochemical properties. GABAA-receptor complex mediates an increase in membrane conductance with an equilibrium potential near the resting level of −70 mV. This conductance increase often is accompanied by a membrane hyperpolarization, which later results in a reduction in the probability of action potential initiation. The reduction in membrane resistance is accomplished by the GABA-dependent facilitation of Cl− ion influx. GABAB receptors are coupled indirectly to K+ channels. When activated, GABAB receptors reduce Calcium ion conductance and inhibit cAMP production via intracellular mechanisms mediated by G-proteins. GABAB receptors are known to mediate both postsynaptic and presynaptic inhibition.

Especificidade

GABA is a neurotransmitter synthesized in all species, and is therefore expected to react to all species.

Recognizes GABA. Staining was blocked by preabsorbing with 100 μM GABA conjugated to glutaraldehyde. 500 μM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining.

Imunogênio

BSA-conjugated GABA-Glutaraldehyde

Aplicação

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GABA in rat brain tissue.

ELISA Analysis: A representative lot from an independent laboratory detected GABA in a competitive ELISA.

Immunohistochemistry Protocol:

Tissues fixed with 4% paraformaldehyde and 0-0.5% glutaraldehyde gives good results. Glutaraldehyde is required for antibody reactivity.

1) Tissue is fixed with 4% paraformaldehyde, 0-0.5% glutaraldehyde, 0.5% potassium dichromate in 0.1M phosphate buffer at pH 6.5.

2) Tissue is post-fixed overnight, vibratome sectioned in 50 mm and incubated in 0.05M Tris buffer, pH 6.5 for three hours.

3) Sections are incubated for 18-24 hours in AB175 diluted in PBS containing 0.1% sodium azide, 0.2% Triton X-100 and 1% normal goat serum.

4) Fluorescein conjugated antibody or ABC system may be used as the secondary reagent.

Note: Without colchicine pretreatment well-stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

This Anti-GABA Antibody is validated for use in Immunohistochemistry (Paraffin) and Enzyme Immunoassay (ELISA) for the detection of GABA.

Qualidade

Evaluated by Immunohistochemistry in rat brain tissue.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected GABA in rat brain tissue.

forma física

Depleted serum

Guinea Pig polyclonal BSA-depleted antiserum with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
Rat Brain tissue

Outras notas

Concentration: Variable

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable