8.51209

D(+)-Biotin

Novabiochem^®

• Sinônimo(s): D(+)-Biotin, D-Biotin,Vitamin H, Hexahydro-2-oxothieno(3,4-d)-imidazole-4-pentanoic acid, Vitamin H
• Fórmula empírica (Notação de Hill): C₁₀H₁₆N₂O₃S
• Número CAS: 58-85-5
• Peso molecular: 244.31
• Número MDL: MFCD00005541
• Código UNSPSC: 41116133
• Número do índice EC: 200-399-3
• NACRES: NA.22

Propriedades

• Nível de qualidade: 200
• linha de produto: Novabiochem^®
• forma: solid
• potência: >2000 mg/kg LD₅₀, oral (Rat)
• adequação da reação: reaction type: Biotinylations
• fabricante/nome comercial: Novabiochem^®
• pH: 4.5 (0.1 g/L in H₂O)
• pf: 232-233 °C
• solubilidade: 0.2 g/L
• densidade volumétrica: 200‑500 kg/m³
• aplicação(ões): peptide synthesis
• temperatura de armazenamento: 2-8°C
• InChI: 1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)
• chave InChI: YBJHBAHKTGYVGT-UHFFFAOYSA-N

Descrição

Descrição geral

D(+)-Biotin is the simplest reagent for the introduction of biotin to peptides, proteins and other biomolecules. In SPPS, it is best coupled using DIPCDI/Oxyma Pure. Biotin-labeled peptides have many important applications in immunology and histochemistry, such as affinity purification and FRET-based flow cytometry, solid-phase immunoassays, and receptor localization, that exploit the high affinity of streptavidin and avidin for biotin.^,,,

Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis

Literature references:
^[1] K. Hofmann & Y. Kiso (1976) Proc. Natl. Acad. Sci., 73, 3516.
^[2] T. Buranda, et al. (1999) Cytometry, 37, 21.
^[3] I. Sélo, et al. (1996) J. Immunol. Methods, 199, 127.
^[4] J. Howl, et al. (1993) Eur J Biochem., 213, 711.

Aplicação

Some recent applications of D(+)-Biotin in SPPS:

• In the high-throughput biotinylation of proteins. The approach for site-specific biotinylation of proteins is useful for various applications such as protein purification and analysis.
• In the site-specific biotinylation of multipodal glycoclusters on a solid support. The synthesis of biotinylated glycoclusters, has important applications in glycobiology.

Informações legais

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable