482728
Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb
liquid, Calbiochem®
Sinônimo(s):
Anti-iNOS
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
serum
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
forma
liquid
contém
≤0.1% sodium azide as preservative
reatividade de espécies
mouse, rat, human
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
avoid repeated freeze/thaw cycles
Isotipo
IgG
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... NOS1(4842)
Descrição geral
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~130 kDa inducible nitric oxide synthase (iNOS) protein.
Recognizes the ~130 kDa iNOS protein.
This Anti-Nitric Oxide Synthase, Inducible Rabbit pAb is validated for use in Frozen Sections, WB, ICC, Paraffin Sections for the detection of Nitric Oxide Synthase, Inducible.
Imunogênio
Mouse
a synthetic peptide (CKKGSALEERKATRL) corresponding to amino acids 1131-1144 of mouse macrophage inducible nitric oxide synthase (iNOS), conjugated to KLH
Aplicação
Frozen Sections (1:500)
Immunoblotting (1:2000)
Immunocytochemistry (1:500)
Paraffin Sections (1:500)
Immunoblotting (1:2000)
Immunocytochemistry (1:500)
Paraffin Sections (1:500)
Advertência
Toxicity: Standard Handling (A)
forma física
Undiluted serum.
Reconstituição
Following initial thaw, aliquot and freeze (-20°C).
Nota de análise
Positive Control
Raw 264.7 cells treated with IFNγ and LPS
Raw 264.7 cells treated with IFNγ and LPS
Outras notas
Exhibits no cross-reactivity with bNOS or eNOS. Variables associated with assay conditions will dictate the proper working dilution.
Recommended Procedure for Immunocytochemistry of Anti-iNOS:
1. Grow cells in appropriate medium.
2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
3. Wash cells 3-5 min with phosphate buffered saline (PBS).
4. Permeabilize with 2% saponin for 10 min at room temperature.
5. Fix in 4% formalin for 10 min at room temperature.
6. Wash 3-5 min with PBS.
7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
8. Wash 3-5 min with PBS.
9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
Recommended Procedure for Immunocytochemistry of Anti-iNOS:
1. Grow cells in appropriate medium.
2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
3. Wash cells 3-5 min with phosphate buffered saline (PBS).
4. Permeabilize with 2% saponin for 10 min at room temperature.
5. Fix in 4% formalin for 10 min at room temperature.
6. Wash 3-5 min with PBS.
7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
8. Wash 3-5 min with PBS.
9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
Lowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA89, 6711.
Lyons, C., et al. 1992. J. Biol. Chem.267, 6370.
Bredt, D.S., et al. 1991. Nature351, 714.
Lyons, C., et al. 1992. J. Biol. Chem.267, 6370.
Bredt, D.S., et al. 1991. Nature351, 714.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Certificados de análise (COA)
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