386032
Anti-Hsp70 Mouse mAb (C92F3A-5)
liquid, clone C92F3A-5, Calbiochem®
Sinônimo(s):
Anti-Heat Shock Protein 70
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
C92F3A-5, monoclonal
forma
liquid
contém
≤0.1% sodium azide as preservative
reatividade da espécie (prevista por homologia)
all
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
avoid repeated freeze/thaw cycles
Isotipo
IgG1
Condições de expedição
wet ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... HSPA1A(3303)
Descrição geral
Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections).
Mouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.
Recognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.
Imunogênio
Hsp70 from HeLa cells
Human
Aplicação
ELISA (see comments)
Flow Cytometry (see comments)
Frozen Sections (5 µg/ml)
Immunoblotting (1 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 µg/ml)
Flow Cytometry (see comments)
Frozen Sections (5 µg/ml)
Immunoblotting (1 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 µg/ml)
Embalagem
Please refer to vial label for lot-specific concentration.
Advertência
Toxicity: Standard Handling (A)
forma física
In PBS, 50% glycerol, pH 7.2.
Reconstituição
Following iniital thaw, aliquot and freeze (-20°C).
Nota de análise
Positive Control
L929 cells, Human colon cancer tissue
L929 cells, Human colon cancer tissue
Outras notas
Does not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Hang, H., and Fox, M.H. 1995. Cytometry19, 119.
Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589.
Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724.
Gower, D.J., et al. 1989. J. Neurosurg.70, 605.
Milarski, K., et al. 1989.J. Cell Biol.108, 413.
Vass, K., et al. 1988. Acta Neuropathologica77, 128.
Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.
Kilgore, J.L., et al. 1994. J. Appl. Physiol.76, 589.
Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab.74, 724.
Gower, D.J., et al. 1989. J. Neurosurg.70, 605.
Milarski, K., et al. 1989.J. Cell Biol.108, 413.
Vass, K., et al. 1988. Acta Neuropathologica77, 128.
Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol.103, 2035.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Aigang Lu et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 22(2), 183-195 (2002-02-02)
Estradiol reduces brain injury from many diseases, including stroke and trauma. To investigate the molecular mechanisms of this protection, the effects of 17-beta-estradiol on heat shock protein (HSP) expression were studied in normal male and female rats and in male
Anne K Voss et al.
The EMBO journal, 25(15), 3652-3663 (2006-07-22)
The mechanisms regulating the size of the cerebral cortex are poorly understood. Here, we demonstrate that the Rap1 guanine nucleotide exchange factor, C3G (Grf2, Rapgef1), controls the size of the cerebral precursor population. Mice lacking C3G show overproliferation of the
Daniel Laubitz et al.
Experimental physiology, 91(5), 867-875 (2006-05-27)
The heat shock response is associated with the intracellular expression of a number of highly conserved heat shock proteins (Hsps). According to their molecular size, Hsps have been divided into several groups, which are strongly conserved and show high homology
Rosario Barranco et al.
International journal of legal medicine, 133(5), 1461-1467 (2019-06-22)
The diagnosis of drowning is one of the most difficult in forensic medicine. The aim of this study was to analyze pulmonary tissue reactions in death by drowning. In particular, we focused on the immunohistochemical expression of P-selectin, SP-A, HSP70
James H Ahn et al.
Assay and drug development technologies, 9(3), 236-246 (2010-12-08)
Heat shock protein 70 (Hsp70) is a chaperone protein that helps protect against cellular stress, a function that may be co-opted to fight human diseases. In particular, the upregulation of Hsp70 can suppress the neurotoxicity of misfolded proteins, suggesting possible
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