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385931

Sigma-Aldrich

Hyaluronidase, Bovine Testes

Sinônimo(s):

Hyaluronidase, Bovine Testes

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About This Item

Número CAS:
Número da licença da enzima:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.54
Preço e disponibilidade não estão disponíveis no momento.

conjugado

conjugate (Glucosaminoglycan)

Nível de qualidade

descrição

Merck USA index - 14, 4758

Formulário

lyophilized

atividade específica

≥2500 units/mg dry wt.

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze

Condições de expedição

ambient

temperatura de armazenamento

−20°C

Descrição geral

Native hyaluronidase from bovine testes. Catalyzes the hydrolysis of 1,4 linkages between N-acetylhexosamine and D-glucuronate residues in chondroitin, chondroitin-4-sulfate, chondroitin-6-sulfate, and hyaluronic acid.
Native hyaluronidase from bovine testes. Hyaluronic acid degrading endoglycosidase that catalyzes the hydrolysis of 1,4 linkages between N-acetylhexosamine and D-glucuronate residues in chondroitin, chondroitin-4-sulfate, chondroitin-6-sulfate, and hyaluronic acid.
Note: 1 KU = 1000 units.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme that will cause the same turbidity reduction as 1.0 unit of International Standard preparation.

Reconstituição

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 week at 4°C.
Reconstitute in 20 mM sodium phosphate, pH 6.9, 0.45% NaCl, and 0.01% BSA.

Outras notas

Lokeshwar, V.B., et al. 2002. J. Biol. Chem.277, 33654.
Humphrey, I.H. 1957. Bull. WHO16, 291.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Declarações de precaução

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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J H HUMPHREY
Bulletin of the World Health Organization, 16(2), 291-294 (1957-01-01)
The International Standard for Hyaluronidase is derived from a blend of four batches of bovine testicular hyaluronidase which had been purified so as to contain approximately 400 units per milligram. The material was mixed with beta-lactose, and lyophilized. Tablets were
Vinata B Lokeshwar et al.
The Journal of biological chemistry, 277(37), 33654-33663 (2002-06-27)
Hyaluronidase is a hyaluronic acid-degrading endoglycosidase that is present in many toxins and the levels of which are elevated in cancer. Increased concentration of HYAL1-type hyaluronidase correlates with tumor progression and is a marker for grade (G) 2 or 3

Artigos

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Questions

1–3 of 3 Questions  
  1. I am doing experiments on hyaluronidase activity and I would like information on how the reconstituted enzyme performs after being frozen and thawed at both -20C and -80C

    1 answer
    1. It is recommended to reconstitute this product in 20 mM sodium phosphate, pH 6.9, 0.45% NaCl, and 0.01% BSA at 5 mg/mL. Following reconstitution, store at 2 - 8°C up to 1 week. Stock solutions may be stored in working aliquots at -20°C for several months. Avoid repeated freeze/thaw cycles.

      Helpful?

  2. What is molecular weight of this product (in kDa)?

    1 answer
    1. The molecular weight is approximately 61 kDa. This information may be found on the Certificate of Analysis.

      Helpful?

  3. We have a bottle of Hyaluronidase, Bovine Testes in our lab and need to know if it has an expiration date. The only date on the bottle is the received date written by one of the techs. If it is stored properly in the freezer what is the expiration date?

    1 answer
    1. This product is not assigned an expiration date or recommended retest date. Customers should routinely inspect products with no expiration date or recommended retest date to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      To discuss further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Report Product Issues" under the Products Section with all the required information, including the lot number, so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

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