1.52028
Chromolith® NH2 (monolithic) HPLC Columns
L × I.D. 100 mm x 4.6 mm HPLC Column
Sinônimo(s):
Chromolith® Performance NH2 100-4.6 HPLC column
About This Item
Produtos recomendados
Nome do produto
Chromolith® NH2, L × I.D. 100 mm x 4.6 mm HPLC column
Materiais
PEEK column
Nível de qualidade
Agency
suitable for USP L8
linha de produto
Chromolith®
Características
endcapped: no
embalagem
1 ea of
Extensão da rotulagem
5% Carbon loading
Parâmetros
200 bar pressure
45 °C max. temp.
técnica(s)
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)
coluna C × D.I.
10 cm × 4.6 mm
Matriz
monolithic silica
Grupo ativo da matriz
NH2 bonding phase
tamanho de poro
13 nm mesoporosity
2 μm macroporosity
Faixa de pH operacional
2-7.5
técnica de separação
hydrophilic interaction (HILIC)
normal phase
reversed phase
temperatura de armazenamento
no temp limit
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Descrição geral
Chromolith® NH2 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® NH2 columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® NH2 columns are suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® NH2 5-4.6 guard cartridges (3 pieces) [1.52030 with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52030]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Nota de análise
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.30 %
Theoretical Plates (N/m) (Cytosine): ≥ 80000
Capacity factor (Cytosine): ≥ 1.3
Informações legais
Certificados de análise (COA)
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Artigos
A new HPLC-RI method is proposed for the analysis of Lactose in milk using a Chromolith® NH2 column.
This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
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