07-1596
Anti-SIRT3 (CT) Antibody
from rabbit
Sinônimo(s):
SIR2-like protein 3 , mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-like 3, sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
reatividade de espécies
human, mouse
técnica(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... SIRT3(23410)
mouse ... Sirt3(64384)
Descrição geral
SIRT3 is a member of the class I sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT3 is located in the mitochondria and has been implicated in regulating metabolic processes. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of Sirt3 in cultured cells increases respiration and decreases the production of reactive oxygen species. Fasting increases Sirt3 expression in white and brown adipose tissue. A longer form of SIRT3 is present in the nucleus where it exhibits histone deacetylase activity. SIRT3 protects cardiomyocytes from stress mediated cell death due to deacetylation of a nuclear factor, Ku-70.
Especificidade
Not tested in other species.
This antibody recognizes SIRT3. Endogenous levels of this protein vary among cell lines; the protein was easily detected in RAW264.7 and C2C12 cells but only weakly detected in HeLa cells, and not detected in Jurkat, HEK293 or PC3 cells.
Imunogênio
Epitope: C-terminus
The immunogen was a peptide corresponding the C-terminal region of human SIRT3.
Aplicação
Anti-SIRT3 (C-terminus) Antibody is a Rabbit Polyclonal Antibody for detection of SIRT3 (C-terminus) also known sirtuin 3 & has been validated in WB, ELISA & IHC.
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).
Immunohistochemistry:
This antibody has been shown by an outside laboratory to be suitable for histochemistry using human hepatocarcinoma tissue (1:50 – 1:100).
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).
Immunohistochemistry:
This antibody has been shown by an outside laboratory to be suitable for histochemistry using human hepatocarcinoma tissue (1:50 – 1:100).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Histone Modifying Proteins
Qualidade
Western Blot Analysis:
1:250 dilution of this antibody detected SIRT3 on 10 µg of C2C12 lysate.
1:250 dilution of this antibody detected SIRT3 on 10 µg of C2C12 lysate.
Descrição-alvo
44 kDa
forma física
Format: Purified
Purified
Purified rabbit polyclonal in buffer containing 0.09% NaN3.
Armazenamento e estabilidade
Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Nota de análise
Control
C2C12 cell lysate
C2C12 cell lysate
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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