04-1506

Anti-CTCF Antibody, clone 1.2.1.5.4

clone 1.2.1.5.4, from mouse

• Sinônimo(s): 11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), CTCFL paralog, transcriptional repressor CTCF
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 1.2.1.5.4, monoclonal
• reatividade de espécies: human
• técnica(s): ChIP: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_006556.1
• nº de adesão UniProt: P49711
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CTCF(10664)

Descrição

Descrição geral

CTCF, also known as CCCTC-binding factor, contains 11 zinc finger (ZF) binding domains that allow it to bind different DNA target sequences and proteins. This multifunctional protein can act as a transcriptional activator or repressor. As a transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mutations of CTCF in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms tumors, suggesting a role for CTCF may as a tumor suppressor.

Especificidade

This antibody recognizes CTCF.

Imunogênio

His-tagged recombinant protein corresponding to human CTCF.

Aplicação

Anti-CTCF Antibody, clone 1.2.1.5.4 is a Mouse Monoclonal Antibody for detection of CTCF also known as 11-zinc finger protein, CCCTC-binding factor & has been validated in WB, IP & IHC.

Chromatin Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory in ChIP. (Pugacheva, E., et al. (2005). Human Molecular Genetics. 14(7):953–965.)

Qualidade

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected CTCF on 10 µg of K562 cell lysate.

Descrição-alvo

~ 130 kDa
Note: Poly(ADP-ribosyl)ated isoform, C-terminal truncation, or sumoylation will cause variable migration of CTCF protein on SDS–PAGE, which may present CTCF with different molecular weight on Western Blot. Modifications are dependent on cell type and growing condition.
The following molecular weights have been reported in the literature: 180 kDa (CTCF-180), 130 kDa (CTCF-130), 82 kDa (CTCF-82), 70 kDa (CTCF-70). See Klenova,E.M.,et al, (1997) Nucleic Acids Res. 25, 466–473 and Torrano,V,et al, (2006) Journal of Cell Science 119, 1746-1759) for additional details.

Ligação

Replaces: MABE547

forma física

Format: Purified

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable