Z699187
MI-Broth Media
pack of 50 × 2 mL
Sinônimo(s):
e coli media, media broth
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About This Item
Código UNSPSC:
41106200
NACRES:
NB.79
Produtos recomendados
embalagem
pack of 50 × 2 mL
fabricante/nome comercial
Maine Mfg 10496192
aplicação(ões)
food and beverages
Descrição geral
MI Broth and MI Agar
MI Broth detects the presence of coliform bacteria by the production of b-galactosidase, which cleaves the substrate MUGal to produce 4-methylumbelliferone, which fluoresces on exposure to UV light. Non-coliforms do not produce this enzyme and therefore do not fluoresce on the medium. Escherichia coli is detected by the compound IBDG. The b-glucuronidase produced by Escherichia coli cleaves the substrate to produce a blue indigo color in the colonies. As Escherichia coli is also a total coliform, and also produces b-galactosidase, it will also fluoresce. The antibiotic cefsulodin is present to inhibit the growth of gram-positive bacteria and some non-coliform gram-negative bacteria that can cause false positive reactions.
MI Broth detects the presence of coliform bacteria by the production of b-galactosidase, which cleaves the substrate MUGal to produce 4-methylumbelliferone, which fluoresces on exposure to UV light. Non-coliforms do not produce this enzyme and therefore do not fluoresce on the medium. Escherichia coli is detected by the compound IBDG. The b-glucuronidase produced by Escherichia coli cleaves the substrate to produce a blue indigo color in the colonies. As Escherichia coli is also a total coliform, and also produces b-galactosidase, it will also fluoresce. The antibiotic cefsulodin is present to inhibit the growth of gram-positive bacteria and some non-coliform gram-negative bacteria that can cause false positive reactions.
Outras notas
Field of Use : For internal research use only. Products are not intended for diagnostic use or resale.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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