748110

SF₇-AM

95% (HPLC)

• Sinônimo(s): SF₇-AM, Bis(acetoxymethyl)
• Fórmula empírica (Notação de Hill): C₃₁H₂₃N₇O₁₂
• Número CAS: 1416872-50-8
• Peso molecular: 685.55
• Código UNSPSC: 12352200
• ID de substância PubChem: 329765872

Propriedades

• Ensaio: 95% (HPLC)
• pf: 160-165 °C
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: CC(OCOC(CN(CC(OCOC(C)=O)=O)C(C1=CC=C(C(OC23C4=C(C=C(N=[N+]=[N-])C=C4)OC5C2C=CC(N=[N+]=[N-])=C5)=O)C3=C1)=O)=O)=O
• InChI: 1S/C31H25N7O12/c1-16(39)45-14-47-27(41)12-38(13-28(42)48-15-46-17(2)40)29(43)18-3-6-21-24(9-18)31(50-30(21)44)22-7-4-19(34-36-32)10-25(22)49-26-11-20(35-37-33)5-8-23(26)31/h3-11,22,25H,12-15H2,1-2H3
• chave InChI: KVUJKFQKNGAJST-UHFFFAOYSA-N

Descrição

Aplicação

SF₇-AM is a fluorogenic; or "caged" rhodamine 110 derivative for the detection of hydrogen sulfide (H₂S). Upon exposure to H₂S; the azide functional groups are reduced to primary amines; restoring the fluorescence of the "caged" fluorophore. A fluorescence intensity increase of 20x is observed upon 1h of SF₇-AM exposure to H₂S. This activation of SF₇-AM has been shown to detect H₂S levels as low as 500 nM in vitro. SF₇-AM has also been engineered specifically for use in live cell imaging of H₂S through the addition of the acetoxymethyl ester groups; which are cleaved in vivo by esterases. This results in adding a net -2 charge to the probe; which was demonstrated to "trap" the probe in cells; thus increasing the sensitivity of the probe even when the cell culture media was changed.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H315,H319,H335
• Declarações de precaução: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable