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SHC016V

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles

Targets no known genes from any species

Synonyme(s) :

MISSION®, MISSION® Control Transduction Particles, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control

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About This Item

Code UNSPSC :
41106609
Nomenclature NACRES :
NA.51

Niveau de qualité

100

Gamme de produits

MISSION®

Concentration

≥1x106 VP/ml (via p24 assay)

Conditions d'expédition

dry ice

Température de stockage

−70°C

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Catégories apparentées

Description générale

The MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Transduction Particles are provided as 200 μL at 1 x 106 TU/mL via p24 assay.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines.

Remarque sur l'analyse

To see more application data, protocols, vector maps visit sigma.com/shrna.

Informations légales

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

En option

Réf. du produit
Description
Tarif

SHC001V

MISSION® pLKO.1-puro Empty Vector Control Transduction Particles, Contains no shRNA insert

SHC002V

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes

SHC003V

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency

SHC007V

MISSION® Luciferase shRNA Control Transduction Particles, shRNA sequence targeting luciferase

SHC012V

MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles, Contains a gene encoding TagRFP

SHC014V

MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Transduction Particles, Contains a gene encoding TurboGFP, under the control of the UbC promoter

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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SIRT6 Depletion Suppresses Tumor Growth by Promoting Cellular Senescence Induced by DNA Damage in HCC.
Lee N
PLoS ONE, 11(11), e0165835-e0165835 (2016)
Namgyu Lee et al.
PloS one, 11(11), e0165835-e0165835 (2016-11-09)
The role of Sirtuin 6 (SIRT6) as a tumor suppressor or oncogene in liver cancer remains controversial. Thus, we identified the specific role of SIRT6 in the progression of hepatocellular carcinoma (HCC). SIRT6 expression was significantly higher in HCC cell
Hedgehog associated to microparticles inhibits adipocyte differentiation via a non-canonical pathway.
Fleury A
Scientific Reports, 6 (2016)
Natsumi Suzuki et al.
Oncogene, 39(10), 2202-2211 (2019-12-13)
p53 is one of the most important tumor suppressor genes, and the exploration of p53-target genes is important for elucidation of its functional mechanisms. In this study, we identified Armadillo Repeat gene deleted in Velo-Cardio-Facial syndrome (ARVCF) as a direct
Jessica B Casaletto et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(15), 7533-7542 (2019-03-23)
Activation of the Met receptor tyrosine kinase, either by its ligand, hepatocyte growth factor (HGF), or via ligand-independent mechanisms, such as MET amplification or receptor overexpression, has been implicated in driving tumor proliferation, metastasis, and resistance to therapy. Clinical development
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