No. The cloning site, EcoR1, is ablated during production to prevent cloning into the vector. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/396/419/shc003bul.pdf
SHC003
MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA
Green fluorescent protein marker to monitor transduction efficiency
Synonyme(s) :
MISSION® Control Vectors
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611,00 €
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1 PKG
611,00 €
About This Item
611,00 €
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Produits recommandés
Gamme de produits
MISSION®
Concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Catégories apparentées
Description générale
The MISSION® TurboGFP™ Control Vector is an 8347 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the CMV promoter. The TurboGFP Control Vector is useful as a positive transfection/transduction control in experiments using the MISSION shRNA library clones.[1][2] TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA has been used to construct GATA2 overexpression vector (oeG2), by removing the GFP cassette of this vector at the 5′ NheI and 3′ PstI cloning sites and replacing it with the full-length GATA2 transcript sequence.[3]
To see more application data, protocols, vector maps visit sigma.com/shrna.
Informations légales
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
En option
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Certificats d'analyse (COA)
Lot/Batch Number
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Articles
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
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dear officer I'd like to know if I'm able to clone in shRNA of choice downstream of the U6 promoter of this plasmid (SHC003)? is there any RE site available to do so? Thank you
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Can I use MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003, to produce lentiviral particles?
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The MISSION® Lentiviral Packaging Mix can be used if you wish to produce virus in your own lab. The product code is SHP001. This product is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene. The Lentiviral Packaging Mix is designed to be co-transfected along with a compatible lentiviral transfer vector (such as SHC003) into HEK293T cells in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. The procedure is available in the Technical Bulletin for product SHP001.
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How much plasmid DNA do I receive when I order MISSION® Control Vector Purified DNA TurboGFP™, Product SHC003?
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Each MISSION Control Vector is provided as 10 μg purified plasmid DNA at a concentration of ~500 ng/μL in 10 mM Tris-HCl, pH 8.0, containing 1 mM EDTA.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What competent cells can I use to propagate the MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003?
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We use GC5 cells (identical genotype to DH5α-T1R) to propagate the Mission shRNA plasmids and controls.
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Can I purchase lentiviral particles produced using MISSION® Control Vector Purified DNA TurboGFP™ plasmid, Product SHC003, directly from Sigma-Aldrich?
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Yes. The product code is SHC003V.
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