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N5264

Sigma-Aldrich

Monoclonal Anti-Neurofilament 160 antibody produced in mouse

clone NN18, ascites fluid

Synonyme(s) :

Monoclonal Anti-Neurofilament medium chain

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About This Item

Numéro MDL:
MFCD01322393
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

NN18, monoclonal

Poids mol.

antigen 160 kDa

Contient

15 mM sodium azide

Espèces réactives

human, pig

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue sections
immunohistochemistry (frozen sections): suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès UniProt

P07197

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

chicken ... NEFM(396206)
human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)

Description générale

Monoclonal Anti-Neurofilament 160 (mouse IgG1 iso-type) is produced by the fusion mouse myeloma cells and splenocytes from an immunized mouse. Neurofilaments are composed of three sub-units- light NFL protein, medium NFM protein, heavy NFH protein. Along with there two other intermediate filaments are present α-internexin and peripherin. Neurofilaments are phosphoproteins.
Neurofilaments belong to the intermediate filament family and are expressed mainly in cells or tissues of neuronal origin. It is crucial for proper radial growth of axons.

Spécificité

Mouse monoclonal anti-neurofilament 160 antibody reacts specifically with neurofilaments of molecular weight 160,000 but does not cross react with other intermediate filament proteins.

Immunogène

neurofilaments from pig spinal cord.

Application

Monoclonal anti-neurofilament 160 antibody (diluted 1: 5000) can be used as internal loading controls in immunoblotting. It can also be used in whole-mount immunohistochemistry and western blot. Monoclonal anti-neurofilament 160 antibodies can be used for localization of neurofilaments of molecular weight 160,000 in cultured cells or tissues preparation.
Monoclonal anti-neurofilament 160 antibody has been used in
  • Immunoblotting.
  • Immunohistochemistry
  • Immunofluorescence

Actions biochimiques/physiologiques

Neurofilaments play a role in axonal calibre as they help in movement of an impulse down the axon. Their activity depends on phosphorylation of neurofilaments.Mutations of neurofilaments causes Charcot-Marie-Tooth (CMT) disease. Accumulation of neurofilaments has been observed in many human neurological diseases like Alzheimer′s disease, progressive supranuclear palsy, diabetic neuropathy, and giant axonal neuropathy.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Svetlana Gorokhova et al.
PLoS genetics, 10(2), e1004081-e1004081 (2014-02-12)
Neurotrophins and their receptors control a number of cellular processes, such as survival, gene expression and axonal growth, by activating multiple signalling pathways in peripheral neurons. Whether each of these pathways controls a distinct developmental process remains unknown. Here we
Peripheral nerve regeneration monitoring using multilayer microchannel scaffolds.
Yoonsu Choi et al.
Neural regeneration research, 11(3), 422-423 (2016-04-30)
F Maina et al.
Genes & development, 11(24), 3341-3350 (1998-02-07)
The development of the nervous system is a dynamic process during which factors act in an instructive fashion to direct the differentiation and survival of neurons, and to induce axonal outgrowth, guidance to, and terminal branching within the target tissue.
Tiziana Bisogno et al.
The Journal of cell biology, 163(3), 463-468 (2003-11-12)
Diacylglycerol (DAG) lipase activity is required for axonal growth during development and for retrograde synaptic signaling at mature synapses. This enzyme synthesizes the endocannabinoid 2-arachidonoyl-glycerol (2-AG), and the CB1 cannabinoid receptor is also required for the above responses. We now
Hak Hyun Ryu et al.
Journal of veterinary science, 10(4), 273-284 (2009-11-26)
In this study, we evaluated if the implantation of allogenic adipose-derived stem cells (ASCs) improved neurological function in a canine spinal cord injury model. Eleven adult dogs were assigned to three groups according to treatment after spinal cord injury by
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