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MBD0015

Sigma-Aldrich

4′,6-Diamidino-2-phénylindole dihydrochloride

DAPI ready made solution

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

Synonyme(s) :

2-(4-Amidinophényl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride

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1 ML
68,00 €
5 ML
320,00 €
10 ML
576,00 €
50 ML
1 880,00 €

68,00 €

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Devis pour commande en gros
Toutes les photos(3)

Sélectionner une taille de conditionnement

Changer de vue
1 ML
68,00 €
5 ML
320,00 €
10 ML
576,00 €
50 ML
1 880,00 €

About This Item

Formule empirique (notation de Hill):
C16H15N5 · 2HCl
Numéro CAS:
28718-90-3
Poids moléculaire :
350.25
Beilstein:
4894417
Numéro MDL:
MFCD00012681
Code UNSPSC :
41105500
Nomenclature NACRES :
NA.55

68,00 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros

Niveau de qualité

200

Forme

liquid

Conditions de stockage

protect from light

Concentration

1 mg/mL

Technique(s)

transfection: suitable

Solubilité

water: soluble

Fluorescence

λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

Adéquation

suitable for nucleic acid staining

Conditions d'expédition

dry ice

Température de stockage

−20°C

Chaîne SMILES 

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

Clé InChI

FPNZBYLXNYPRLR-UHFFFAOYSA-N

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Catégories apparentées

Description générale

DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.

Application

Le DAPI est nettement plus sensible que le bromure d′éthidium pour marquer l′ADN dans les gels d′agarose. Il peut être utilisé dans le photofootprinting de l′ADN, pour détecter les sondes hybridées dans les applications sur membranes en permettant la visualisation spécifique des complexes double-brins, et pour évaluer les changements de l′ADN et de son contenu lors de l′apoptose par cytométrie en flux. La coloration par DAPI est également une méthode sensible et spécifique de détection des mycoplasmes.
DAPI Readymade Solution is provided at 1mg/mL concentration. It is recommended to dilute DAPI readymade solution 1:1000 with a desired buffer for a 1μg/mL working concentration (typical working concentration used in most applications is 0.1μg/mL-10μg/mL). Store DAPI solution (1mg/mL) -20°C protected from light. It is recommended to aliquot DAPI readymade solution and to avoid freeze thaw cycles.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).

Actions biochimiques/physiologiques

Sonde fluorescente pour l′ADN qui se lie dans le petit sillon, capable de pénétrer dans les cellules. Se lie au petit sillon d′ADN double brin (préférentiellement à l′ADN riche en AT), formant un complexe stable dont l′intensité de fluorescence est environ 20 fois plus élevée que celle du DAPI non lié.
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Caractéristiques et avantages

  • Ready-to-use liquid, cell permeable dye.
  • Can be used with live cells and fixed cells.
  • Effective for fixed-cell staining and quantitation of DNA content.
  • Optimal Counterstain when using fluorescent antibodies during fluorescence microscopy or high-content screening (HCS) applications.

Forme physique

DAPI readymade solution is provided at 1mg/mL concentration in water

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000380386
0000190381
0000216561
0000367284
0000187804

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

June Zhang et al.
Chemosphere, 256, 127038-127038 (2020-05-30)
Baicalein is a flavonoid that is widely found in plants. Studies have shown that baicalein has anti-inflammatory, anti-cancer, and liver-protective effects. However, the effects of baicalein on TAA-induced toxicity and the underlying molecular mechanisms in zebrafish larvae are still unknown.
Mark Jones et al.
Cell transplantation, 29, 963689720923578-963689720923578 (2020-07-15)
Absence or reduced frequency of human regulatory T cells (Tregs) can limit the control of inflammatory responses, autoimmunity, and the success of transplant engraftment. Clinical studies indicate that use of Tregs as immunotherapeutics would require billions of cells per dose.
Maksim A Nesterenko et al.
Parasites & vectors, 13(1), 559-559 (2020-11-11)
Parasitic flatworms (Trematoda: Digenea) represent one of the most remarkable examples of drastic morphological diversity among the stages within a life cycle. Which genes are responsible for extreme differences in anatomy, physiology, behavior, and ecology among the stages? Here we report
Wen Zeng et al.
Frontiers in cellular neuroscience, 15, 638351-638351 (2021-03-09)
Brain microvascular endothelial cells (BMECs) are a major component of the blood-brain barrier that maintains brain homeostasis. Preserving and restoring the normal biological functions of BMECs can reverse or reduce brain injury. Endothelial progenitor cells (EPCs) may promote brain vascular
Vasileios Kampanis et al.
Cells, 10(1) (2021-01-01)
The central nervous system (CNS) does not recover from traumatic axonal injury, but the peripheral nervous system (PNS) does. We hypothesize that this fundamental difference in regenerative capacity may be based upon the absence of stimulatory mechanical forces in the
Afficher tous les articles scientifiques apparentés
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Questions

1–2 of 2 Questions  

  1. What concentration should be use for live/dead cell staining for live cells in FACS sorter, to be able to culture back the sorted cells?

    1 answer

    1. The optimum concentration varies, depending on the cell type. However, a concentration of 0.1-1.0 µg/mL is generally sufficient for live/dead FACS sorting. Titrate DAPI to determine the concentration best suited for the cell type.

      The following paper with protocols that may be useful for live/dead FACS sorting:
      https://onlinelibrary.wiley.com/doi/10.1002/eji.201646632

      Helpful?

  2. Is this product sterile?

    1 answer

    1. Product MBD0015 is not a sterile. This solution is at a 1 mg/mL concentration. Required volumes may be diluted with PBS or water and filter sterilized if desired.

      Helpful?

Reviews

Active Filters

  1. Egypt
    • Review 1
    • Votes 0
    1 out of 5 stars.

    The product is not working on frozen sections

    I bought this product to stain frozen sections however the product did not stain frozen sections. The R and D team did not have specific recommendation.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to call or email us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

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