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H7787

Millipore

HIS-Select® Spin Columns

Synonyme(s) :

His-Tag Spin Columns

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10 EA
183,00 €
50 EA
606,00 €

183,00 €

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10 EA
183,00 €
50 EA
606,00 €

About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

183,00 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.

Durée de conservation

2 yr at 2‑8 °C

Conditionnement

pkg of 10 ea
pkg of 50 ea

Dimension de pores

100 nm pore size

Capacité

>0.5 mg/column

Température de stockage

2-8°C

Catégories apparentées

Description générale

Kit contains 3 collection tubes for every spin column.

Application

Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.

Caractéristiques et avantages

  • High Selectivity for higher purity
  • Convenient and Ready-to-Use
  • Consistent protein binding each use
  • Compatible with CelLytic™ cell lysis reagents

Informations légales

CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000437261
0000437261
SLCQ6970
SLCQ5845
SLCL7069

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Maria A Schumacher et al.
Nucleic acids research, 46(14), 7405-7417 (2018-06-16)
Streptomyces are filamentous bacteria with a complex developmental life cycle characterized by the formation of spore-forming aerial hyphae. Transcription of the chaplin and rodlin genes, which are essential for aerial hyphae production, is directed by the extracytoplasmic function (ECF) σ
Tian Luo et al.
Clinical and vaccine immunology : CVI, 17(1), 87-97 (2009-12-04)
Recently, major species-specific antibody epitopes in three immunoreactive tandem repeat proteins (TRPs) of Ehrlichia chaffeensis, TRP32, TRP47, and TRP120, have been identified and molecularly characterized within tandem repeat (TR) regions. In this study, we mapped the major immunodeterminants of the
Michael D BeGora et al.
The Journal of biological chemistry, 285(38), 29147-29155 (2010-07-24)
Three sequential methylations of phosphoethanolamine (PEA) are required for the synthesis of phosphocholine (PCho) in plants. A cDNA encoding an N-methyltransferase that catalyzes the last two methylation steps was cloned from Arabidopsis by heterologous complementation of a Saccharomyces cerevisiae cho2
Dirk Maass et al.
PloS one, 4(7), e6373-e6373 (2009-07-29)
As the first pathway-specific enzyme in carotenoid biosynthesis, phytoene synthase (PSY) is a prime regulatory target. This includes a number of biotechnological approaches that have successfully increased the carotenoid content in agronomically relevant non-green plant tissues through tissue-specific PSY overexpression.
Anne Durand et al.
The Journal of biological chemistry, 281(40), 29558-29567 (2006-07-26)
In Escherichia coli, the ammonia channel AmtB and the P(II) signal transduction protein GlnK constitute an ammonium sensory system that effectively couples the intracellular nitrogen regulation system to external changes in ammonium availability. Binding of GlnK to AmtB apparently inactivates
Afficher tous les articles scientifiques apparentés

Questions

1–3 of 3 Questions  

  1. Can HIS-Select® Spin Column, Product H7787, be re-used?

    1 answer

    1. We do not recommend re-using the columns. Each column is intended for one time use.

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  2. What is the binding capacity of the HIS-Select® Spin Column, Product H7787?

    1 answer

    1. The binding capacity of a HIS-Select Spin Column is >500 ug per column as determined witha ~30 kDa histidine tagged protein recovered under high levels of protein expression.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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