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H2153

Sigma-Aldrich

HABA/Avidin Reagent

lyophilized powder

Synonyme(s) :

HABA Reagent

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Forme

lyophilized powder

Niveau de qualité

200

Utilisation

sufficient for ≤10 assays

Couleur

orange

Adéquation

suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples

Température de stockage

2-8°C

Description générale

4-Hydroxyazobenzene-2-carboxylic acid (HABA) is an avidin-binding dye that can be stoichiometrically displaced by biotin. The subsequent change in absorption at A500 provides a basis for spectrophotometric determination of biotin levels in biological samples.
HABA/Avidin Reagent is specific for chromogenic applications that depend upon quantification of biotin.

Application

HABA/Avidin Reagent is suitable for spectrophotometric determination of biotin and biotin conjugates in a wide variety of samples.
Reagent is capable of detecting biotin at μMolar concentrations (i.e., 1 nmole per ml). Typically used to quantitate biotin in biotinylated compounds.

Reconstitution

Prepares a ready-to-use HABA/avidin solution in buffered saline, pH approx. 7.3, with preservative when reconstituted with 10 ml deionized water.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Mentions de danger

H412

Conseils de prudence

P273 - P501

Classification des risques

Aquatic Chronic 3

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

A Einspanier et al.
Biology of reproduction, 61(2), 512-520 (1999-07-20)
Relaxin is a peptide hormone with a broad range of biological activities, related not only to parturition and lactation but possibly also to decidualization, implantation, and early pregnancy. The present study was designed to investigate the secretion pattern of relaxin
R S Niedbala et al.
Journal of biochemical and biophysical methods, 13(4-5), 205-210 (1986-11-01)
Parameters and conditions of an enzyme based assay for biotin and avidin are presented. Biotinylated glucose-6-phosphate dehydrogenase when complexed with avidin becomes inactivated. Thus it was possible to construct a competitive assay system for biotin. The assay is sensitive between

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