BK101
ImmunoProbe™ Biotinylation Kit
Synonyme(s) :
Biotinylation Kits
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About This Item
Code UNSPSC :
41116134
eCl@ss :
34058011
Nomenclature NACRES :
NA.32
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Température de stockage
2-8°C
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Description générale
For custom biotinylation of proteins, Sigma offers kits for conjugation on two different scales. The biotin-avidin system has become popular when high sensitivity and specificity are desired. Biotin can be conjugated to antibodies, lectins, enzymes, and other proteins. The protocols in the kits have been optimized for antibodies. Avidin binds to biotin with a high affinity (Ka = 1015 M) and specificity. When conjugated to enzymes or fluorochromes avidin provides a means of identifying biotinylated compounds via enzymatic conversion of substrate to form a visible product or detection of fluorescence by spectrophotometry or flow cytometry.
Application
ImmunoProbe™ Biotinylation Kit has been used for preparation of biotin labeled antibodies.
Biotin has been modified with aminocaproate, then activated via an ester linkage with sulfo-N-hydroxysuccinimide (BAC-Sulfo-NHS). Aminocaproate provides a six-carbon spacer that reduces steric hindrance on the biotin and improves accessibility to the binding site on avidin. Sulfonation of the hydroxysuccinimide increases the polarity of the reagent, allowing it to dissolve easily in aqueous buffer. The ester provides a carbonyl carbon adjacent to a labile ester linkage as a target for primary amine side chains of accessible lysine residues, joining the biotinamidocaproate to the protein via an amide bond.
Caractéristiques et avantages
- Complete protocols for labeling and assay
- BAC-Sulfo-NHS is completely water soluble
- No DMF or DMSO needed
- Biotinylation occurs near neutral pH and physiological ionic strength, avoiding harsh conditions that could damage sensitive proteins
- Fast separation of conjugate from reactants using gel filtration
- 2-5 molar ratio biotin to protein in conjugate (for antibodies)
1 mg protein per reaction (B-TAG)
10 mg protein per reaction (BK-101)
• Sufficient reagents for at least 5 labelings
Conditionnement
Kit includes all reagents necessary for biotinylation and for determination of biotin/protein molar ratio.
Remarque sur l'analyse
Procedure:
Conjugation is performed in four easy steps:
1. Reconstitute BAC-Sulfo-NHS with Phosphate Buffer (PB).
2. Add BAC-Sulfo-NHS to protein and allow to react for 30 minutes at room temperature.
3. Separate the conjugate from the reactants on gel filtration.
4. Assay the conjugate for biotin incorporation by the avidin-HABA assay (BK-101 only). Conjugate is ready to use.
Conjugation is performed in four easy steps:
1. Reconstitute BAC-Sulfo-NHS with Phosphate Buffer (PB).
2. Add BAC-Sulfo-NHS to protein and allow to react for 30 minutes at room temperature.
3. Separate the conjugate from the reactants on gel filtration.
4. Assay the conjugate for biotin incorporation by the avidin-HABA assay (BK-101 only). Conjugate is ready to use.
Informations légales
ImmunoProbe is a trademark of Sigma-Aldrich Co. LLC
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
10 - Combustible liquids
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Contenu apparenté
Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.
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