C4879

α-Chymotrypsinogen A from bovine pancreas

essentially salt-free, lyophilized powder

• Synonyme(s) : chymotrypsin A zymogen
• Numéro CAS: 9035-75-0
• Numéro CE : 232-905-3
• Numéro MDL: MFCD00130483
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bovine pancreas
• Type: Type II
• Forme: essentially salt-free, lyophilized powder
• Activité spécifique: ≥40 units/mg solid
• Poids mol.: 25,656 Da by calculation
• Produit purifié par: 6× crystallization
• Solubilité: 1 mM HCl: soluble 10 mg/mL, clear, colorless
• Numéro d'accès UniProt: P00767
• Activité étrangère: α-chymotrypsin ≤1 U/mg (prior to activation by trypsin)
• Température de stockage: −20°C
• Informations sur le gène: cow ... CTRB1(618826)

Description

Description générale

Chymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges.[1] It has an isoelectric pH of 8.97.

Application

α-Chymotrypsinogen A from bovine pancreas has been used as model protein crystallization reproducibility studies.[2] It has also been used in the hydrolysis of α-gliadins prior to mass spectroscopy studies.[3]

The enzyme from Sigma has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy.[4] It has been used to study the partitioning of protein in polymer/polymer aqueous two-phase systems.[5] The enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution.[6]

Actions biochimiques/physiologiques

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond.

Chymotrypsinogen A requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form m α, β, γ, δ and π chymotrypsin (depending upon the conditions of activation).[7] Chymotrypsin is a protease that will preferentially cleave peptides on the carboxyl side of aromatic amino acids including tryptophan, tyrosine, and phenylalanine. It will also hydrolyze peptides on the carboxyl side of leucine, methionine, and alanine.[8]

Définition de l'unité

After activation to Chymotrypsin, one unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Autres remarques

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)