B3670

Betacellulin human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

• Synonyme(s) : BTC
• Numéro CAS: 163150-12-7
• Numéro MDL: MFCD00676521
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Pureté: ≥97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 0.1-0.6 ng/mL ED₅₀
• Poids mol.: protein 9.5 kDa
• Conditionnement: pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: P35070
• Température de stockage: −20°C
• Informations sur le gène: human ... BTC(685)

Description

Application

This product has been used to study differentiation of pancreatic acinar cell line (AR42J) into insulin-secreting cells and has been linked to a role in the growth and differentiation of islet cells in human pancreas.

Actions biochimiques/physiologiques

Betacellulin, and the other members of the EGF family of growth factors, binds with high affinity to both EGFR and to erbB-4. Overexpression of Betacellulin with erbB-3 and erbB-4 has been linked to human endometrial adenocarcinoma.

Attention

This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.

Notes préparatoires

This product was lyophilized from a 0.2 μm filtered solution of PBS containing 5 mg of bovine serum albumin. It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 1 μg/mL.

Remarque sur l'analyse

The biological activity of this product is measured by an ability to stimulate H-thmidine incorporation in EGF-responsive mouse BALB/c 3T3 fibroblasts. The EC₅₀ is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)