11426320001
Roche
Anti-Fluorescein
from mouse IgG1 (clone B13-DE1)
Synonyme(s) :
antibody
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About This Item
Code UNSPSC :
12352200
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
B13-DE1, monoclonal
Forme
lyophilized
Conditionnement
pkg of 100 μg
Fabricant/nom de marque
Roche
Isotype
IgG1
Température de stockage
2-8°C
Description générale
Monoclonal antibody to fluorescein.
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.
Contents
Lyophilizate, stabilized
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.
Contents
Lyophilizate, stabilized
Spécificité
The monoclonal antibody reacts with free and bound fluorescein.
Application
Use Anti-Fluorescein for the detection of fluorescein-labeled compounds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
Notes préparatoires
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.
- ELISA: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.
Reconstitution
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Reconstitute for 15 minutes.
Reconstitute for 15 minutes.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3 - Skin Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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