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556881

Sigma-Aldrich

Ribonuclease Inhibitor, Human, Recombinant, E. coli

The Ribonuclease Inhibitor, Human, Recombinant, E. coli controls the biological activity of Ribonuclease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

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About This Item

Code UNSPSC :
12352200

Niveau de qualité

Forme

liquid

Activité spécifique

≥20,000 units/mL

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Activité étrangère

Endonuclease and RNase, none detected

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.
Recombinant, human ribonuclease inhibitor expressed in E. coli. Non-competitive inhibitor that inactivates RNase by non-covalent binding. Has been used to improve cDNA synthesis and in vitro RNA synthesis, increase yields of polysomes, and aid in the preparation of RNase-free antibodies. Inhibits RNases A, B, and C. Does not inhibit RNase T1 and S1 nuclease from Aspergillus.

Actions biochimiques/physiologiques

Cell permeable: no
Primary Target
inactivates RNase
Product does not compete with ATP.
Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of material that will inhibit 50% of the activity of 5 ng of RNase A.

Forme physique

In 50 mM KCl, 20 mM HEPES, 8 mM DTT, 50% glycerol.

Reconstitution

Maintain at -20°C. Avoid freezing solutions. Addition of DTT is recommended to maintain 8 mM level.

Autres remarques

Saxena, S.K., et al. 1992. J. Biol. Chem. 267, 21982.
Lee, F.S., et al. 1989. Biochemistry 28, 225.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Vera Thole et al.
BMC genomics, 20(1), 995-995 (2019-12-21)
Flavonoids are produced in all flowering plants in a wide range of tissues including in berry fruits. These compounds are of considerable interest for their biological activities, health benefits and potential pharmacological applications. However, transcriptomic and genomic resources for wild
Nida Ali et al.
Nucleic acids research, 48(2), 847-861 (2019-12-06)
RNase E is a 472-kDa homo-tetrameric essential endoribonuclease involved in RNA processing and turnover in Escherichia coli. In its N-terminal half (NTH) is the catalytic active site, as also a substrate 5'-sensor pocket that renders enzyme activity maximal on 5'-monophosphorylated
Asimina Katsiani et al.
The Journal of general virology, 101(6), 676-684 (2020-05-08)
Rose rosette virus (RRV) (genus Emaravirus) is the causal agent of the homonymous disease, the most destructive malady of roses in the USA. Although the importance of the disease is recognized, little sequence information and no full genomes are available
Susara Madduma Hewage et al.
Frontiers in medicine, 7, 80-80 (2020-04-16)
Chronic low-grade inflammation is a major stimulus for progression of chronic kidney disease (CKD) in individuals consuming high-fat diet. Currently, there are limited treatment options for CKD other than controlling the progression rate and its associated complications. Lingonberry (Vaccinium vitis-idaea
Ihshan Akthar et al.
Reproduction (Cambridge, England) (2019-12-04)
We previously reported that sperm binding to cultured monolayers of bovine uterine epithelial cells induces an acute inflammatory response involving the Toll-like receptor (TLR2) signaling pathway. This response serves to clear the uterus of sperm and thereby prepares the endometrium

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