R1158

Ribonuclease Inhibitor

recombinant, expressed in E. coli, 20-40 units/μL

• Synonyme(s) : RNAse inhibitor
• Numéro MDL: MFCD00132187
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.52

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 300
• Qualité: for molecular biology
• Forme: buffered aqueous solution
• Poids mol.: ~50 kDa
• Concentration: 20-40 units/μL
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).

Application

Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:

• cDNA synthesis from mRNA
• reverse transcriptase polymerase chain reaction (RT-PCR)
• in vitro transcription and translation
• mRNA protection from degradation[1]
• formaldehyde cross-linked lncRNA purification[2]
• RNA-fluorescence in situ hybridization (FISH)[3]

Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

Actions biochimiques/physiologiques

Inhibits RNase by forming a tight, non-covalent 1:1 complex.

Composants

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

Définition de l'unité

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

Forme physique

Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.

Autres remarques

• Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
• This product is for R&D use only, not for drug, household, or other uses.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable