ZRB1324

Anti-APOL1 Antibody, clone 1L15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Apolipoprotein L1;Apolipoprotein L;Apo-L;ApoL;Apolipoprotein L-I
• UNSPSC-Code: 12352203
• NACRES: NA.43

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Rekombinant: expressed in HEK 293 cells
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 1L15, recombinant monoclonal
• Beschreibung: recombinant, expressed in HEK 293 cells
• Produktlinie: ZooMAb^® learn more
• Form: lyophilized
• Mol-Gew.: calculated mol wt 43.97 kDa; observed mol wt ~45 kDa
• Aufgereinigt durch: using Protein A
• Speziesreaktivität: human
• Verpackung: antibody small pack of 25 μL
• Grünere Alternativprodukt-Eigenschaften: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Methode(n): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG
• Epitopsequenz: N-terminal half
• Protein-ID-Hinterlegungsnummer: NP_001130012
• UniProt-Hinterlegungsnummer: O14791
• Grünere Alternativprodukt-Kategorie: Aligned,
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... APOL1(8542)

Beschreibung

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spezifität

Clone 1L15 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Apolipoprotein L1 (APOL1). It targets an epitope within 17 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of Human Apolipoprotein L1 (APOL1).

Anwendung

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Apolipoprotein L1 (APOL1) in HepG2 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected His/GST-tagged recombinant Human Apolipoprotein L1 (APOL1) and APOL1 in Human kidney tissue lysates.

Affinity Binding Assay: A representative lot of this antibody bound recombinant Human Apolipoprotein L1 (APOL1) with a KD of 4.0 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Apolipoprotein L1 (APOL1) in Human liver tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Zielbeschreibung

Apolipoprotein L1 (UniProt: O14791; also known as Apolipoprotein L, Apo-L, ApoL, Apolipoprotein L-I, ApoL-I) is encoded by the APOL1 (also known as APOL) gene (Gene ID: 8542) in human. APOL1 is a membrane-associated protein with multiple transmembrane domains and localizes to multiple cellular membrane environments. It is mainly synthesized in the liver with a signal peptide (aa 1-27) and released into circulation where it circulates in complex with HDL particles. It probably plays a role in lipid exchange and transport throughout the body and may participate in reverse cholesterol transport from peripheral cells to the liver. In kidney, it is expressed in podocytes and glomerular and extraglomerular vascular endothelial cells. Mutations in APOL1 gene have been linked to nondiabetic kidney disease and focal segmental glomerulosclerosis (FSGS4) that is characterized by reduced glomerular filtration rate and progressive decline in renal function leading to end-stage renal disease. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Madhavan, SM., and Buck, M. (2021). Kidney 360. 2(1); 134-140; Friedmann, DJ., and Pollak, MR (2016). Trends Endocrinol. Metab. 27(4); 204-215).

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstituierung

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable