TP0010

FLAG^® HA Tandem Affinity Purification Kit

Name: FLAG<SUP>®</SUP> HA Tandem Affinity Purification Kit
Brand: SIGMA
Price: 867 EUR

Synonym(e):

Anti-ddddk, Anti-dykddddk

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Über diesen Artikel

NACRES:

NA.32

UNSPSC Code:

12352200

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storage temp.

2-8°C

Quality Level

200

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Dieser Artikel	FLAGIPT1	F2426	A2220
Millipore TP0010 FLAG^® HA Tandem Affinity Purification Kit	Millipore FLAGIPT1 FLAG^® Immunoprecipitation Kit	Millipore F2426 EZview^™ Red ANTI-FLAG^® M2 Affinity Gel	Millipore A2220 ANTI-FLAG^® M2 Affinity Gel
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200

General description

TAP technology is a recent development in protein purification. This technology incorporates tandem-linked affinity tags into genes of interest so that high purity fusion proteins can be isolated through two consecutive affinity purification steps. The technology is particularly useful for the isolation and identification of protein complexes by adding a tandem affinity tag to a known "bait" protein to pull down endogenous proteins that interact with the targeted protein of interest.

Application

The FLAG^® HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and tissue homogenates. The kit is particularly suitable for isolation of protein complexes using TAP (Tandem Affinity Purification) technology.

Sutitable for mass spectrometry analysis and western blotting.

Learn more product details in our FLAG^® application portal.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.

Beschreibung

SDB

F2426EZview^™ Red ANTI-FLAG^® M2 Affinity Gel, clone M2SDB

A2095Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspensionSDB

R0278RIPA BufferSDB

F47993X FLAG^® Peptide, lyophilized powderSDB

U4883Urea, 8 M (after reconstitution with 16 mL high purity water)SDB

Lagerklasse

10 - Combustible liquids

wgk

WGK 3

pictograms

GHS07

signalword

Danger

hcodes

H319,H412,EUH430

pcodes

P201 - P202 - P273 - P391 - P405 - P501

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2

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FLAG® HA System

The FLAG® HA tandem epitope system consists of small epitopes tags that are not eukaryotic derived. These features minimize interference with protein functions and provide superior specificity.

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