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F2426

Millipore

EZview™ Red ANTI-FLAG® M2-Affinitätsgel

clone M2

Synonym(e):

Monoklonaler ANTI-FLAG® M2-Antikörper in Maus hergestellte Antikörper, Anti-ddddk, Anti-dykddddk

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise

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1 ML
€ 822,00

€ 822,00

Voraussichtliches Versanddatum17. März 2025

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1 ML
€ 822,00

About This Item

UNSPSC-Code:
12352203
NACRES:
NA.32

€ 822,00

Voraussichtliches Versanddatum17. März 2025

Bulk-Bestellung anfordern

Klon

M2, monoclonal

Qualitätsniveau

200

chemische Klasse(n) des Analyten

proteins

Methode(n)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

Matrix

4% agarose bead; 45-165μm bead size

Isotyp

IgG1

Kapazität

≥0.6 mg/mL, gel binding capacity

Versandbedingung

wet ice

Lagertemp.

−20°C

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Verwandte Kategorien

Allgemeine Beschreibung

Das EZview Red Anti-FLAG M2-Affinitätsgel ist ein Harz, das aus Anti-FLAG M2-Antikörpern besteht, die kovalent an Agarose-Beads (4 % Agarose) gebunden sind. Das Affinitätsgel wird verwendet, um FLAG-Fusionsproteine an Proben wie Zelllysate und Gewebe zu binden und FLAG-getaggte Proteine zur Vorbereitung für Immunpräzipitations-Assays aufzureinigen. Der rote Farbstoff sorgt für eine bessere Sichtbarkeit und effizientere Ergebnisse. Agarose-Beads binden an N-terminalen, Met-N-terminalen, C-terminalen FLAG-Fusionsproteinen und 3xFLAG-getaggten Fusionsproteinen.

Spezifität

Geeignet für die Aufreinigung von N-terminalen, Met-N-terminalen, C-terminalen FLAG-Fusionsproteinen und 3xFLAG-Fusionsproteinen.

Anwendung

Immunpräzipitation (IP) von FLAG- und 3xFLAG-markierten Fusionsproteinen.

Elution – FLAG-Peptid, Glycin, pH 3,5, 3xFLAG-Peptid

Weitere Produktinformationen finden Sie in unserem FLAG® Literatur-Portal.

Physikalische Form

1:1 (v/v) Suspension in PBS enthält 50 % Glycerin und 15 ppm Kathon

Rechtliche Hinweise

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

FLAG™-Affinitätsgele, FLAG™-Tag, 3xFLAG™-Tag, DYKDDDDK-Tag

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06693

Timestrip Plus™ -20 °C

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Kara R Jones et al.
Molecular cancer therapeutics, 4(10), 1541-1547 (2005-10-18)
Incomplete DNA repair or misrepair can contribute to the cytotoxicity of DNA double-strand breaks. Consequently, interference with double-strand break repair, by pharmacologic or genetic means, is likely to sensitize tumor cells to ionizing radiation. The current studies were designed to
Matthew S Walters et al.
Journal of virology, 84(13), 6861-6865 (2010-04-16)
Varicella zoster virus encodes an immediate-early (IE) protein termed ORF61p that is orthologous to the herpes simplex virus IE protein ICP0. Although these proteins share several functional properties, ORF61p does not fully substitute for ICP0. The greatest region of similarity
W Du et al.
Cell death and differentiation, 16(11), 1493-1504 (2009-07-11)
The tumor suppressor p53 induces potent anti-proliferative responses in stressed cells; in unstressed cells this ability of p53 is restrained by Hdm2. Expression of Hdm2 is also induced by p53, thereby establishing feedback inhibition. Regulation of the p53-Hdm2 interaction and
Jiahai Zhou et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(39), 14343-14348 (2006-09-16)
The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1) and PKR-related ER kinase (PERK) are two type I
Annie M Sriramachandran et al.
Nature communications, 10(1), 3678-3678 (2019-08-17)
Modification with SUMO regulates many eukaryotic proteins. Down-regulation of sumoylated forms of proteins involves either their desumoylation, and hence recycling of the unmodified form, or their proteolytic targeting by ubiquitin ligases that recognize their SUMO modification (termed STUbL or ULS).
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Protokolle

Immunpräzipitation von FLAG-Fusionsproteinen mit Affinitätsgelen monoklonaler Antikörper

Protokoll für die Immunpräzipitation (IP) von FLAG-Fusionsproteinen unter Einsatz des monoklonalen M2-Antikörper-Affinitätsgels (4 % Agarose)

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Verwandter Inhalt

Proteinaufreinigung

Proteinaufreinigungsverfahren, Reagenzien und Protokolle zur Aufreinigung rekombinanter Proteine unter Anwendung von Methoden wie Ionenaustausch, Größenausschluss und Proteinaffinitätschromatographie.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Proteinexpression

Technologien zur Expression rekombinanter Proteine in E. coli-, Insekten-, Hefen- und Säuger-Expressionssystemen für die Grundlagenforschung und zur Unterstützung der Therapeutika- und Impfstoffproduktion.

Protein Expression

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

Questions

1–6 of 6 Questions  

  1. What is the binding capacity of the Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, resin?

    1 answer

    1. The binding capacity of the resin must be   ? 0.6 mg/mL to meet specifications.  This capacity will vary from lot to lot.

      Helpful?

  2. When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, should I use a 3X FLAG peptide or a 1X FLAG peptide to elute my protein?

    1 answer

    1. If you have a 3X FLAG-tagged protein, then you will need to use the 3X FLAG peptide.  If you have a 1X FLAG-tagged protein, you can use the 1X FLAG peptide or the 3X FLAG peptide.  We have not noticed a significant  difference in elution efficiency by using a 3X FLAG peptide on a 1X FLAG-tagged protein.

      Helpful?

  3. When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, I see bands at 20-25 kDa and 50-60 kDa appearing in my Westerns that are not my FLAG-tagged protein. How can I prevent this?

    1 answer

    1. As a result of the conjugation, there may be some M2 antibody that is not conjugated to the resin, but is associated with the resin and may appear in acid elutions as heavy and light chain when using the anti-mouse IgG conjugated secondary antibody.  We recommend a acid wash (0.1 M glycine-HCL pH 3.5) and neutralization of the resin (do not allow the acid wash to sit on the resin longer than 20 minutes) prior to applying the lysate.  Another way to avoid this is to use a directly conjugated FLAG antibody for detection such as product A8592 ant-FLAG M2 HRP, or the rabbit anti-FLAG polyclonal antibody, F7425.

      Helpful?

  4. When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, I have a lot of non-specific proteins that are eluting with my FLAG-tagged protein. How can I get rid of these?

    1 answer

    1. One way to remove non-specific proteins is to pre-bind the protein lysate with unconjugated resin.  We recommend product 4B200 for this purpose. Other methods would be to increase the stringency of the washes by increasing salt concentration (the resin can tolerate up to 1M NaCl) or including detergents that are compatible with the resin.

      Helpful?

  5. When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, how can I elute my protein?

    1 answer

    1. Elution with the peptide is the most gentle method.  Acid elution (0.1 M glycine-HCL pH 3.5) is a more stringent method of elution, and should be evaluated for its effect on your protein if it is to be used in downstream applications.  Boiling the resin in sample buffer is the most denaturing condition.  If this condition is used, the resin cannot be re-used, due to the presence of SDS and/or reducing agents.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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