SRP0280

UbcH13 (UBE2N) Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonym(e): UBC13 homolog, UBE2N, UbcH13, Ubiquitin-conjugating enzyme E2N
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥80% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 17.6 kDa
• Verpackung: pkg of 100 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_003348
• UniProt-Hinterlegungsnummer: P61088
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... UBE2N(7334)

Beschreibung

Allgemeine Beschreibung

UbcH13 (GenBank Accession No. NM_003348), amino acids 2-end, with N-terminal His-tag, MW = 17.6 kDa, expressed in an E. coli cell expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.