SAB4200776

Anti-Tubulin, Tyrosine antibody, Mouse monoclonal

clone TUB-1A2, purified from hybridoma cell culture

• Synonym(e): Tubulin Alpha-1 Chain
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: TUB-1A2, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: ~55 kDa
• Speziesreaktivität: Xenopus, Arabidopsis sp., yeast, insect, rat, hamster, porcine, monkey, bovine, human, mouse, canine, chicken
• Konzentration: ~1 mg/mL
• Methode(n): immunoblotting: suitable; immunofluorescence: 2-4 μg/mL using human HeLa or Chicken fibroblast cells; immunohistochemistry: suitable
• Isotyp: IgG3
• UniProt-Hinterlegungsnummer: Q71U36
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... TUBA1A(7846)

Beschreibung

Allgemeine Beschreibung

Anti-Tubulin, Tyrosine antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the TUB-1A2 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse. Tubulin is an intracellular cylindrical filamentous structure and is presented in eukaryotic cells. It consists of three isoforms α-, β- and γ-tubulin. Tubulin is a heterodimer of α- and β- tubulin subunits.

Spezifität

Anti-Tubulin, Tyrosine antibody, Mouse monoclonal specifically recognizes the C-terminal Tyrosine of Tubulin protein. The antibody is non-reactive with cells that have been treated with pancreatic carboxypeptidase A under conditions which remove only the C-terminal tyrosine. Monoclonal Anti-Tubulin, Tyrosine recognizes Tubulin from human, mouse, chicken, bovine, rat, hamster, canine, monkey, porcine, arabidopsis, tobacco, yeast, Xenopus, insect, comb jelly, sponge, and worm origin.

Immunogen

a synthetic peptide containing the carboxy terminal amino acids of porcine a-tubulin

Anwendung

Anti-Tubulin, Tyrosine antibody, mouse monoclonal may be used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Biochem./physiol. Wirkung

Tubulin is the major building block of microtubules which is essential for structural and mobility elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. It is also involved in the nervous system. Tubulin is associated with neurodegenerative disorders such as Alzheimer′s and Parkinson′s disease. Mutations in the αβ- dimers and γ-tubulin are associated with malformation and impairment of cognitive disorders.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Haftungsausschluss

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable