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S0266

Spermidin

BioReagent, Molecular Biology, suitable for cell culture, ≥98%

Synonym(e):

1,8-Diamino-4-azaoctan, N-(3-Aminopropyl)-1,4-diaminobutan

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Ihnen/SKUVerfügbarkeitPreis
1 g
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€ 73,30
5 g
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€ 254,00
25 g
Warenkorb auf Verfügbarkeit prüfen
€ 790,00

Über diesen Artikel

Lineare Formel:
NH2(CH2)3NH(CH2)4NH2
CAS-Nummer:
Molekulargewicht:
145.25
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
EC Number:
204-689-0
Beilstein/REAXYS Number:
1698591
MDL number:
Assay:
≥98%
Form:
solid or liquid

€ 73,30


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grade

Molecular Biology

Quality Segment

product line

BioReagent

assay

≥98%

form

solid or liquid

mol wt

145.25 (AMU)

technique(s)

cell culture | mammalian: suitable

refractive index

n20/D 1.479 (lit.)

density

0.925 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, and protease, none detected

storage temp.

room temp

SMILES string

NCCCCNCCCN

InChI

1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2

InChI key

ATHGHQPFGPMSJY-UHFFFAOYSA-N

Gene Information

rat ... Ppm1a(24666)

General description

Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.

Application

Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.
Spermidine has been used in the preparation of gold beads and as a component in nuclei isolation buffer (NIB).

Biochem/physiol Actions

Hemmt die neuronale Stickoxidsynthase (nNOS). Bindet und fällt DNA aus; kann zur Aufreinigung von DNA-Bindungsproteinen verwendet werden. Stimuliert die T4-Polynukleotidkinaseaktivität.
Hemmt die neuronale Stickoxidsynthase (nNOS). Bindet und fällt DNA aus; stimuliert die T4-Polynukleotidkinaseaktivität.
Spermidine helps to maintain the proper activity of gene expression. This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300). Spermidine participates in cell growth.

Disclaimer

Für eine sterile Lösung soll die Lösung steril-filtriert und nicht autoklaviert werden. Spermidin deaminiert mit der Zeit; Lösungen tiefgekühlt lagern. Regelmässig frische Lösungen zubereiten.

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Dieser Artikel
85558S262649761
Spermidin analytical standard

Supelco

49761

Spermidin

description

BioReagent, Molecular Biology, suitable for cell culture, ≥98%

description

BioUltra, Molecular Biology, ≥99.5% (GC)

description

≥99% (GC)

description

analytical standard

form

solid or liquid

form

-

form

solid or liquid (clear)

form

-

assay

≥98%

assay

≥99.5% (GC)

assay

≥99% (GC)

assay

≥99.0% (GC), ≥97.5% (NT)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

technique(s)

cell culture | mammalian: suitable

technique(s)

buffer exchange: suitable, ChIP: suitable

technique(s)

-

technique(s)

gas chromatography (GC): suitable, HPLC: suitable

mol wt

145.25 (AMU)

mol wt

-

mol wt

-

mol wt

-

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

2-8°C


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pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Lagerklasse

8A - Combustible corrosive hazardous materials

flash_point_f

233.6 °F - closed cup

flash_point_c

112 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter



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Analysenzertifikate (COA)

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Questions

1–7 of 7 Questions  
  1. Can the reconstituted S0266 solution, which has been sitting at room temperature for 3 months, still be used in CUT&Tag assays to stabilize nuclei? Also, how can the integrity of spermidine be tested? If there's no more spermidine available to test the solution, should more be purchased?

    1 answer
    1. The integrity of Spermidine can be verified by Gas chromatography. The following publication may be of interest for protocol reference on Gas chromatography analysis of Spermidine.
      1. J Mass Spectrom. 2009 Aug;44(8):1203-10. doi: 10.1002/jms.1597.
      2. A quantitative GC-MS method for three major polyamines in postmortem brain cortex, PMID: 19514045

      Helpful?

  2. What is the solvent that spermidine is dissolved in?

    1 answer
    1. This product is a neat or pure liquid and has not been diluted in a solvent. However, to further dilute this product, it is recommended to use water at 50 mg/mL water.

      Helpful?

  3. How to store S0266-5G? which comes as liquid and what is the molaritty of it? which solvent to use to dilute it?

    1 answer
    1. This product can be stored in room temperature in its original format. The concentration of this product can be determined using the lot specific purity reported on the Certificate of Analysis, the density, and the compound molecular weight. This product has a density of 0.925 g/mL and a molecular weight of 145.25 g/mol. The minimum purity is 98%, so the molarity will vary slightly from lot to lot. The molarity can range from 6.2M (98%) to 6.4M (100%).
      Molarity=Density x 1000 x Purity/MW

      This product is supplied as a liquid or solid. A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter.

      Helpful?

  4. How to store S0266-1G which comes as liquid? alliquoted and store at -20C? How long it can be stored at this condition?

    1 answer
    1. Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.

      Helpful?

  5. How frequently should new solutions be made?

    1 answer
    1. A stock solution can be made by dissolving 1.45g in 10mL of water followed by sterile filtration with a 0.22um filter. Solutions should be stored as single-use aliquots at -20 ºC for no more than one month.

      Helpful?

  6. Is this a spermidine solution or a neat liquid, and what is the concentration?

    1 answer
    1. Based on a density of 0.925 g/mL and a molecular weight of 145.25, this neat liquid has an effective molarity of 6.4 M.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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