After dispensing, briefly flush the vial headspace with dry nitrogen or argon and cap immediately. The headspace may be cleared by directing the inert gas across the opening of the vial or bottle. Alternatively, handling in an inert-gas enclosure, such as a glove box or Aldrich® AtmosBag (Z530212), is also suitable. Prepared 1 M solutions are stable for 3 to 6 months when stored refrigerated at 2 -8 °C and up to 12 months at - 20 °C, when stored in single use aliquots. Avoid repeated freeze/thaw cycles. While there is no specific guidance on clearing the headspace for prepared solutions, this additional measure would further ensure stability.
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85558
Spermidin
BioUltra, Molecular Biology, ≥99.5% (GC)
Synonym(e):
SPD, 1,8-Diamino-4-azaoctan, N-(3-Aminopropyl)-1,4-diaminobutan
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 g | Warenkorb auf Verfügbarkeit prüfen | € 64,60 |
5 g | Warenkorb auf Verfügbarkeit prüfen | € 224,00 |
Über diesen Artikel
Lineare Formel:
NH2(CH2)3NH(CH2)4NH2
CAS-Nummer:
Molekulargewicht:
145.25
UNSPSC Code:
12352116
NACRES:
NA.26
PubChem Substance ID:
EC Number:
204-689-0
Beilstein/REAXYS Number:
1698591
MDL number:
Assay:
≥99.5% (GC)
Technischer Dienst
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Unterstützung erhaltengrade
Molecular Biology
Quality Segment
product line
BioUltra
assay
≥99.5% (GC)
storage condition
(Tightly closed. Keep locked up or in an area accessible only to qualified or authorizedpersons.)
technique(s)
ChIP: suitable, buffer exchange: suitable
impurities
DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
refractive index
n20/D 1.479 (lit.)
pH
12.0-13.5 (25 °C, 1 M in H2O)
solubility
H2O: 1 M at 20 °C, clear, colorless
density
0.925 g/mL at 25 °C (lit.)
anion traces
chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg
cation traces
Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
λ
1 M in H2O
SMILES string
NCCCCNCCCN
UV absorption
λ: 260 nm Amax: 0.1, λ: 280 nm Amax: 0.05
suitability
suitable for molecular biology
application(s)
advanced drug delivery
genomic analysis
InChI
1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
InChI key
ATHGHQPFGPMSJY-UHFFFAOYSA-N
Gene Information
rat ... Ppm1a(24666)
General description
Spermidin ist ein Polyamin und ein trivalentes organisches Kation.Spermidin ist eine natürlich vorkommende Polyaminverbindung mit verschiedenen biochemischen Eigenschaften. Es spielt eine wichtige Rolle bei der Genexpression und ist an DNA-Replikation, -Transkription und -Translation beteiligt. Spermidin ist von entscheidender Bedeutung für Zellwachstum, -proliferation und -differenzierung und hat Auswirkungen auf die Progression des Zellzyklus und die Gewebeentwicklung. Es besitzt eine antioxidative Aktivität und schützt die Zellen vor oxidativem Stress, indem es freie Radikale abfängt. Spermidin stimuliert auch die Autophagie und fördert den Abbau von geschädigten Organellen und Proteinaggregaten für die zelluläre Homöostase. Mit neuroprotektiven Effekten unterstützt es das neuronale Überleben, die synaptische Plastizität und schützt vor neurodegenerativen Erkrankungen. Darüber hinaus moduliert Spermidin zelluläre Signalwege, die an Entzündungen und Immunreaktionen beteiligt sind.
Application
Spermidin wird wie folgt verwendet:
- als Komponente von Chromosom-Isolationspuffer
- als Komponente von Transkriptionspuffer
- als Komponente von Homogenisierungspuffer
Biochem/physiol Actions
Hemmt die neuronale Stickoxidsynthase (nNOS). Bindet und fällt DNA aus; kann zur Aufreinigung von DNA-Bindungsproteinen verwendet werden. Stimuliert die T4-Polynukleotidkinaseaktivität.
Hemmt die neuronale Stickoxidsynthase (nNOS). Bindet und fällt DNA aus; stimuliert die T4-Polynukleotidkinaseaktivität.
Spermidin moduliert verschiedene Zellaktivitäten wie Zellwachstum und -entwicklung, Stabilität von DNA und Apoptose.
Features and Benefits
- Vielseitig und anpassbar an eine Vielzahl von Labor- und Forschungsanwendungen
- Geeignet für die Forschung in den Bereichen Molekularbiologie und Biochemie
- Frei von DNase, RNase, NICKase und Protease
- In Tests bestätigte niedrige Schwermetallkontamination zur Sicherstellung der Eignung für verschiedene Anwendungen.
- Getestet auf Spuren von Anionen und Kationen
Analysis Note
flüssig oder fest
Other Notes
Füllen Sie bitte dieses Formular aus, um weitere Informationen zu unserem Sortiment an Biochemikalien zu erhalten.
Bei der Erststrang-cDNA-Synthese; Wirkung auf die Endonukleasehemmung durch Agarose-Verunreinigungen[1]
Disclaimer
Für eine sterile Lösung soll die Lösung steril-filtriert und nicht autoklaviert werden. Spermidin deaminiert mit der Zeit; Lösungen tiefgekühlt lagern. Regelmässig frische Lösungen zubereiten.
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Dieser Artikel | |||
|---|---|---|---|
| grade Molecular Biology | grade - | grade Molecular Biology | grade - |
| assay ≥99.5% (GC) | assay ≥99% (GC) | assay ≥98% | assay ≥97% |
| technique(s) ChIP: suitable, buffer exchange: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) - |
| suitability suitable for molecular biology | suitability - | suitability - | suitability - |
| storage condition (Tightly closed. Keep locked up or in an area accessible only to qualified or authorizedpersons.) | storage condition - | storage condition - | storage condition - |
| impurities DNases, none detected, insoluble matter, passes filter test, proteases, none detected, RNases, none detected, phosphatases, none detected | impurities DNase, none detected, RNase, none detected, protease, none detected | impurities - | impurities - |
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signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Lagerklasse
8A - Combustible corrosive hazardous materials
flash_point_f
233.6 °F - closed cup
flash_point_c
112 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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How should one provide inert gas atmosphere for storage after opening the provided vial? Do aliquots (diluted to 1M) need to be kept under inert gas as well? How long can one store said aliquots without impairng activity?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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What's the molar concentration of the product? Is it 1M?
1 answer-
This product is considered a pure material rather than a solution. The concentration can be determined by dividing the density by the molecular weight and correcting for units. This gives 6.37M, which would then need to be multiplied by the batch-specific density for the batch in question, as the material is not 100% pure.
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What is the container for item 85558-1G Spermidine and 43816-10mL DL-Dithiothreitol solution?
1 answer-
Both items are from Buchs. Item 85558-1g comes in a narrow neck glass bottle, 5 ml, amber. Item 43816-10mL is a 15 ml plastic tube made of polypropylene (PP) and includes a sterile cap.
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Under which conditions should this UNopened product be stored? What is the shelf life of an UNopened product? Under which conditions should this OPENED product be stored? What is the shelf life of an OPENED product?
1 answer-
This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. No change in storage temperature is required after opening provided that the item is handled properly and free of contaminants. This product is not assigned an expiration date. While considered stable, this item may be subject to degradation over time and is assigned a periodic retest date to confirm quality. The lot specific retest date is reported on the Certificate of Analysis. See the link below to review a sample of lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/85558#product-documentationHelpful?
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spermidine 85558. I have received 1 G in liquid. Can you please tell me 1G in what volume? and what solution? Never mentioned anywhere.
1 answer-
The product is a neat or pure compound with a melting point of approximately room temperature. The material may be received as either a liquid or a solid. The density of this product is 0.925 g/mL and the concentration is 6.4M.
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Is this a spermidine solution or a neat liquid, and what is the concentration?
1 answer-
Based on a density of 0.925 g/mL and a molecular weight of 145.25, this neat liquid has an effective molarity of 6.4 M.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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