Upon the addition of crystalline serum albumin (10 mg/mL) and storage at -15°C, solutions of this product in water are stable for several months. Additional information can be found in the Methods in Enzymology XIV, 202 (1969).
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P8398
Phospholipase D aus Kohl
Type IV, lyophilized powder, ≥100 units/mg solid
Synonym(e):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase, Phospholipase D from white cabbage
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
500 units | Warenkorb auf Verfügbarkeit prüfen | € 207,00 |
2500 units | Warenkorb auf Verfügbarkeit prüfen | € 647,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-639-8
MDL number:
Specific activity:
≥100 units/mg solid
€ 207,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltentype
Type IV
Quality Segment
form
lyophilized powder
specific activity
≥100 units/mg solid
composition
Protein, ~25%
storage temp.
−20°C
General description
Application
Phospholipase D (PLD) is used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD is used in purification and kinetic studies.
Biochem/physiol Actions
The amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD. It hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Analysis Note
Protein determined using biuret.
Other Notes
One unit will liberate 1.0 μmol of choline from L -α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.
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Dieser Artikel | |||
|---|---|---|---|
| description Type IV, lyophilized powder, ≥100 units/mg solid | description Type VII, lyophilized powder, ≥150 units/mg solid | description lyophilized powder, ≥1,000 units/mg protein (Lowry) | description Type I, lyophilized powder, 10-50 units/mg protein |
| specific activity ≥100 units/mg solid | specific activity ≥150 units/mg solid | specific activity ≥1,000 units/mg protein (Lowry) | specific activity 10-50 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| type Type IV | type Type VII | type - | type Type I |
| composition Protein, ~25% | composition - | composition - | composition protein, 20-40% |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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How long will it remain stable after reconstitution at 4 degrees Celsius?
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