Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
P4649
Phosphorylase b aus Kaninchenmuskel
For use as a marker in SDS-PAGE
Synonym(e):
α-Glucanphosphorylase, 1,4-α-D-Glucan:orthophosphat-α-D-glucosyltransferase, Glykogenphosphorylase
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-737-0
MDL number:
Fortfahren mit
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenSuchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| form powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt observed mol wt ~97 kDa | mol wt - | mol wt 97,200 Da by calculation | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| packaging vial of 0.5 mg | packaging - | packaging - | packaging - |
General description
Phosphorylase b is a dimer, usually exists in inactive form in the skeletal muscles. Equilibrium exists between an active relaxed (R) state and a less active tense (T). Phosphorylase b favors the T state. The enzyme possesses three domains, the N-terminal domain, glycogen-binding domain and the C-terminal domain.
Application
Phosphorylase b from rabbit muscle is to be used as a marker in SDS-PAGE. Phosphorylase b is used during chemical cross-linking studies as a SDS-PAGE molecular weight standard[1][2].
Phosphorylase b from rabbit muscle has been used as a molecular weight marker in 12% polyacrylamide gel for keratinase, and stress-70 protein.
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
B Chen et al.
The Journal of biological chemistry, 275(45), 34946-34953 (2000-08-17)
The envelope glycoprotein, gp160, of simian immunodeficiency virus (SIV) shares approximately 25% sequence identity with gp160 from the human immunodeficiency virus, type I, indicating a close structural similarity. As a result of binding to cell surface CD4 and co-receptor (e.g.
Crystallographic studies on the activity of glycogen phosphorylase b
Weber IT, et al.
Nature, 274(5670), 433-433 (1978)
Signal Transduction and Protein Phosphorylation (2012)
Stress-70 proteins in marine mussel Mytilus galloprovincialis as biomarkers of environmental pollution: a field study
Hamer B, et al.
Environment International, 30(7), 873-882 (2004)
C Donnet et al.
The Journal of biological chemistry, 276(10), 7357-7365 (2000-12-02)
Thermal denaturation can help elucidate protein domain substructure. We previously showed that the Na,K-ATPase partially unfolded when heated to 55 degrees C (Arystarkhova, E., Gibbons, D. L., and Sweadner, K. J. (1995) J. Biol. Chem. 270, 8785-8796). The beta subunit
-
What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
-
-
Has the amino acid sequence of phosphorylase from rabbit muscle been published?
1 answer-
The sequence has been published. The amino acid composition is:Asp5l, Asn45, Thr35, Ser29, Glu64, Gln31, Pro36, Gly48, Ala63, Cys9, Val62, Met2l, Ile49, Leu79, Tyr36, Phe38, Trp12, Lys48, HiS22, and Arg63. Reference for the sequence and amino acid composition: K. Titani et al., Proc. Nat. Acad. Sci., USA, 74, 4762-4766 (1977) (see attached pdf).Product P1261 is phosphorylase a, which is the tetrameric form. Products P4649 and P6635 are phosphorylase b, the dimeric form. The sequence information of the subunit would be the same for both forms.
Helpful?
-
Active Filters
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung