This product can be dissolved in cold deionized water up to 1000 units per mL. The units per mg are batch-dependent and can be found in the Certificate of Analysis.
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P3026
Pektolyase aus Aspergillus japonicus
lyophilized powder, ≥0.3 units/mg solid
Umweltfreundlichere Alternative
Synonym(e):
Pectolytic enzyme
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Specific activity:
≥0.3 units/mg solid
Biological source:
Aspergillus sp. (A. japonicus)
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenbiological source
Aspergillus sp. (A. japonicus)
Quality Segment
form
lyophilized powder
specific activity
≥0.3 units/mg solid
mol wt
50 kDa
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
, Enabling
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.
Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.
Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.
Application
Pectolyase from Aspergillus japonicus has been used:
- in the lysis of chlorella cells for protoplast generation
- as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation
- to induce reorganization of Arabidopsis root cap cell architecture
Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis.
Zur Herstellung von Pflanzenprotoplast für den Zellwandabbau vor der organellen Isolation.
Biochem/physiol Actions
Pectolyase is used for protoplast isolation from plant cells. It also mediates ethylene hormone synthesis.
Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, endo-pectin lyase and a maceration stimulating factora.
Other Notes
1 U setzt 1.0 μmol Galacturonsäure aus Polygalacturonsäure pro Minute frei bei pH 5.5 und 25 °C.
Für weitere Informationen siehe enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.
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| description lyophilized powder, ≥0.3 units/mg solid | description BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry) | description powder, 0.3-3.0 unit/mg solid (using a β-galactose dehydrogenase system and locust bean gum as substrate) | description lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose |
| specific activity ≥0.3 units/mg solid | specific activity ≥5 units/mg protein (Lowry) | specific activity 0.3-3.0 unit/mg solid (using a β-galactose dehydrogenase system and locust bean gum as substrate) | specific activity 30-60 units/mg protein (biuret) |
| biological source Aspergillus sp. (A. japonicus) | biological source Aspergillus niger | biological source - | biological source - |
| form lyophilized powder | form aqueous glycerol solution | form powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 50 kDa | mol wt - | mol wt - | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How to dissolve the lyophilized powder of P3026 coded pectolyase powder? and how to prepare 0.8% solution of it?
1 answer-
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Hello! I want to ask how much cellulase does the pectolyase (P3026) contains? It does not mention any contamination of other enzymes in the product description, but I want to know if there is any potential contamination involved.
1 answer-
This product is not tested for the presence of contaminating enzymes. The primary components are endo-polygalacturonase and endo-pectin lyase. A brief search of the literature shows that in protoplast isolation, cellulase is often added to the digest, in addition to pectolyase.
Helpful?
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Hello, I wanted to ask if this enzyme is equivalent to pectolyase Y-23, as the activity values are expressed differently.
1 answer-
Yes, this product is equivalent to pectolyase Y-23.
Helpful?
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