The concentration in U/mL can be determined using the Enzymatic Activity and the mg protein/mL value reported on the lot specific Certificate of Analysis. For example, a lot has a reported Enzymatic Activity of 359 units/ mg protein and 13.7 mg protein/ mL will have a concentration 4918.3 units/ml. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/US/en/product/sigma/p1506#product-documentation
P1506
Pyruvat-Kinase aus Kaninchenmuskel
Type II, ammonium sulfate suspension, 350-600 units/mg protein
Synonym(e):
ATP:pyruvate 2-O-phosphotransferase, PK, Phospho-enolpyruvat-Kinase
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1000 UNITS
€ 44,60
5000 UNITS
€ 137,00
25000 UNITS
€ 528,00
50000 UNITS
€ 787,00
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
MDL number:
Specific activity:
350-600 units/mg protein
Biological source:
rabbit muscle
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Unterstützung erhaltenbiological source
rabbit muscle
type
Type II
form
ammonium sulfate suspension
specific activity
350-600 units/mg protein
mol wt
237 kDa
storage condition
(Tightly closed)
technique(s)
ligand binding assay: suitable
color
white
foreign activity
lactic dehydrogenase, creatine phosphokinase, and myokinase ≤0.01%, phosphoglucomutase ≤0.05%
storage temp.
2-8°C
Quality Level
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1 of 4
Dieser Artikel | |||
|---|---|---|---|
| biological source rabbit muscle | biological source human | biological source human | biological source rabbit muscle |
| technique(s) ligand binding assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| specific activity 350-600 units/mg protein | specific activity - | specific activity - | specific activity 350-600 units/mg protein |
| form ammonium sulfate suspension | form aqueous solution | form aqueous solution | form lyophilized powder |
| mol wt 237 kDa | mol wt 59 kDa | mol wt 63 kDa | mol wt 237 kDa |
| storage temp. 2-8°C | storage temp. −70°C | storage temp. −70°C | storage temp. −20°C |
General description
Research Area: Cell Signaling
Pyruvate kinase from rabbit muscle catalyzes ATP-dependent phosphorylation of glycolate to yield 2-phosphoglycolate.[1]Pyruvate kinase, an enzyme,[2] is found in a tetrameric or a dimeric form.[3] PKM1, PKM2, PKR, and PKL are the four mammalian pyruvate kinase isoforms.[2]
Pyruvate kinase from rabbit muscle catalyzes ATP-dependent phosphorylation of glycolate to yield 2-phosphoglycolate.[1]Pyruvate kinase, an enzyme,[2] is found in a tetrameric or a dimeric form.[3] PKM1, PKM2, PKR, and PKL are the four mammalian pyruvate kinase isoforms.[2]
Application
Biochem/physiol Actions
Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Pyruvate kinase from rabbit muscle can be activated by histidine and inhibited by low levels of zinc (Zn2+). [6] In the glycolytic pathway, pyruvate kinase (PK) functions as a terminal enzyme, catalyzing the conversion of phosphoenolpyruvate to pyruvate and the synthesis of ATP through substrate-level phosphorylation. Tetrameric structures of PK are more active and have a high affinity for phosphoenolpyruvate (PEP), whereas dimeric structures are less active and have a low affinity for PEP.[3] PK from rabbit muscle possesses positive kinetic cooperativity (Hill coefficient> 1.35) of the phosphoenol pyruvate and adenosine diphosphate(ADP) binding sites.[7]
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH 6
Analysis Note
Protein determined by biuret.
Other Notes
One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
7783-20-2
CAS No.
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Xun Chen et al.
Cancer cell international, 20(1), 523-523 (2020-12-10)
Pyruvate kinase is a terminal enzyme in the glycolytic pathway, where it catalyzes the conversion of phosphoenolpyruvate to pyruvate and production of ATP via substrate level phosphorylation. PKM2 is one of four isoforms of pyruvate kinase and is widely expressed
D E Ash et al.
Archives of biochemistry and biophysics, 228(1), 31-40 (1984-01-01)
Pyruvate kinase from rabbit muscle catalyzes an ATP-dependent phosphorylation of glycolate to yield 2-phosphoglycolate (F. J. Kayne (1974) Biochem. Biophys. Res. Commun. 59, 8-13). An investigation of anologous reactions with other alpha-substituted carboxylic acids reveals several new substrates for such
Glucokinase in Atlantic Halibut (Hippoglossus hippoglossus) Brockmann Bodies
Tranulis MA, et al.
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 116, 367-370 (1997)
Inhibitory effect of Zn2+ on rabbit muscle pyruvate kinase and reactivation by histidine.
Tamaki N.
J. Nutr. Sci. Vitaminol., 27, 107-116 (1981)
S Strumilo et al.
Zhurnal evoliutsionnoi biokhimii i fiziologii, 51(2), 103-107 (2015-06-02)
Some catalytic and kinetic properties of pyruvate kinase (PK, EC 2.7.1.40) isolated from the heart and skeletal muscles of rabbits and hares with a 9-16-fold purification were studied. The initial specific activity of the enzyme in hare heart homogenates was
Artikel
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Verwandter Inhalt
Product Information Sheet
-
この製品(P1506, ピルビン酸キナーゼ)についてご質問があります。 この製品の比活性は350-600 units/mg proteinと記載ありますが、濃度としてU/mLはお分かりになるのでしょうか。 Lot毎に異なることかもしれませんので、試薬の瓶そのものに記載があったりするのでしょうか(データシートには記載が見当たらない)。 ご確認のほど、よろしくお願いいたします。
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Hi, How long can this be stored in the fridge?
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This product is not assigned an expiration date or retest date. Products that are robust in nature and extremely stable, in the original unopened container, are not assigned an expiration date or retest schedule. These products will have no dating reported on either the label or the Certificate of Analysis. Products that are not assigned an expiration or retest date are covered by a warranty period of 1 year from the date of product shipment.
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