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MAK113

Sigma-Aldrich

ATPase/GTPase-Aktivitäts-Assay-Kit

sufficient for 200 colorimetric tests

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1 KIT
€ 476,00

€ 476,00

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1 KIT
€ 476,00

About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

€ 476,00

Versand in der Regel in 2 Wochen.
Bulk-Bestellung anfordern

Verwendung

sufficient for 200 colorimetric tests

Nachweisverfahren

colorimetric

Relevante Krankheit(en)

cancer

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

ATPasen und GTPasen katalysieren die Aufspaltung von ATP oder GTP in ADP oder GDP und freies Phosphat. Diese Enzyme spielen eine Schlüsselrolle beim Transport, der Signaltransduktion, der Proteinbiosynthese und der Zelldifferenzierung.

Leistungsmerkmale und Vorteile

Kompatibel mit Handhabungssystemen mit hohem Durchsatz.

Eignung

Geeignet für den Nachweis von ATPase- und GTPase-Aktivität und für das Screening von ATPase- und GTPase-Inhibitoren.

Prinzip

Das ATPase-/GTPase-Aktivitäts-Assay-Kit bietet ein einfaches, direktes Verfahren für die Messung der ATPase-/GTPase-Aktivität in einem Mikroplattenformat. Dieses Kit verwendet eine einzige Reagenzformulierung zur genauen Bestimmung der Enzymaktivität in 30 Minuten bei Raumtemperatur. Das malachitgrüne Reagenz bildet eine stabile dunkelgrüne Farbe mit freiem Phosphat, das von den Enzymen freigesetzt wird, was zu einem kolorimetrischen Produkt führt, das bei 620 nm (600–660 nm) gemessen wird und proportional zur vorhandenen Enzymaktivität ist. Eine Aktivitätseinheit ist die Enzymmenge, die die Produktion von 1 μMol freiem Phosphat pro Minute unter den Testbedingungen katalysiert.

Piktogramme

Corrosion
GHS05

Signalwort

Warning

H-Sätze

H290

P-Sätze

P234 - P390

Gefahreneinstufungen

Met. Corr. 1

Lagerklassenschlüssel

8B - Non-combustible corrosive hazardous materials

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
113CE07A23
113CE07A23
113CE07A12
113CE02A21
113CE07A12

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

Suche nach COA

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Shannon E Hill et al.
PloS one, 12(7), e0180241-e0180241 (2017-07-26)
Dihydroneopterin triphosphate pyrophosphatase (DHNTPase), a member of the Mg2+ dependent Nudix hydrolase superfamily, is the recently-discovered enzyme that functions in the second step of the pterin branch of the folate biosynthetic pathway in E. coli. DHNTPase is of interest because
Meng-Yan Sun et al.
Oncology reports, 39(6), 2673-2680 (2018-04-06)
Regulator of G‑protein signaling 1 (RGS1) has been found to be a critical factor in melanoma and other malignancies. However, the mechanism involved in the RGS1‑mediated promotion of melanoma progression is not clear. We based our study on samples collected from
Shuhui Wang et al.
Protein & cell, 11(2), 124-137 (2019-11-24)
Type VII secretion systems (T7SSs) are found in many disease related bacteria including Mycobacterium tuberculosis (Mtb). ESX-1 [early secreted antigen 6 kilodaltons (ESAT-6) system 1] is one of the five subtypes (ESX-1~5) of T7SSs in Mtb, where it delivers virulence
Wenjing Jiang et al.
Scientific reports, 8(1), 9706-9706 (2018-06-28)
The resistance to sorafenib highly affects its clinical benefits for treating hepatocellular carcinoma (HCC). Sodium orthovanadate (SOV) is a phosphate analog that displays anti-cancer activities against various types of malignancies including HCC. The present study has demonstrated that SOV is
Shu Yan Liu et al.
Oncotarget, 7(13), 16282-16296 (2016-02-27)
Increased integrin β4 (ITGB4) level is accompanied by malignant progression of multiple carcinomas. However, selective therapeutic strategies against cancer cells expressing a high level of ITGB4 have not been reported. Here, for the first time, we report that a chiral
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Questions

1–6 of 6 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is the MAK113 ATPase/GTPase Activity Assay Kit compatible in both acidic and basic pH environments? Within what pH range is this assay compatible? I am attempting to measure the GTPase activity within a pH range of 5.5 - 8.0.

    1 answer

    1. As per the guidance, the assay has been optimized with Assay Buffer at pH 7.5. It's important to note that the assay is pH dependent, and the enzymatic reaction is stopped by adding an acidic reagent. With the samples themselves expected to be largely neutralized by the assay buffer, they should be suitable within the pH range of 5.5 to 8. However, it is not advisable to prepare a different buffer to test the reaction under more acidic conditions. There is a possibility that conducting the reaction under more acidic conditions, like pH 5.5, might inhibit enzyme activity.

      Helpful?

  4. Does malachite green react with phosphate molecules in which one oxygen is substituted by sulfur when using GTPgammaS instead of GTP to detect RAN GTPase activity? Additionally, could malachite green turn blue in reaction with other molecules besides phosphate?

    1 answer

    1. Unfortunately, it has not been tested whether malachite green also reacts with phosphate molecules in which one oxygen is substituted with sulfur, and there is uncertainty about other molecules that could cause a blue color change. It is also possible that there might be contaminating free phosphate in the materials used for the assay. The datasheet for the kit contains instructions on how to check for any contaminating free phosphate.

      Helpful?

  5. Would storage of the MAK113 ATPase/GTPase Activity Assay Kit at -20°C for 4 days affect its performance?

    1 answer

    1. Based on available information, it is not expected that storage at -20°C would significantly affect the kit’s performance or shelf life.

      Helpful?

  6. What is the detailed composition of Malachite Green Reagent (MAK 113A) in the ATPase/GTPase Activity Assay Kit (MAK113)?

    1 answer

    1. A detailed composition of the reagent is not available as this is considered proprietary information.

      Helpful?

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